通过核基因ITS2片段研究四种鲇形目鱼类进化

采用鲇形目鱼鱼类通用引物扩增了鲇形目鱼类4种鱼,长吻(Leiocassi longirostris)、南方南方大口鲇(Si-lurus meridionalis)、黄颡鱼(Pseudobagrus fulvidraco)、斑点叉尾(Ictalurus punctatus)核DNA的ITS2片段,并构建UPMGA,NJ,ME和MP系统树。结果显示:根据遗传距离,斑点叉尾和黄颡鱼的亲缘关系最近(D=0.0661),接下来是黄颡鱼和南方大口鲇(D=0.1100),南方大口鲇和长吻(D=0.1184),斑点叉尾和南方大口鲇(D=0.1266),黄颡鱼和长吻(D=0.1490),斑点叉尾和长吻的亲缘关系是最远的(D=0.1503)。结果表明:长吻最早分化,其次是南方南方大口鲇和黄颡鱼,而斑点叉尾分化最晚。

Evolution Relationship on Siluriformes using ITS2 Fragments Analysis

Four species Samples of Siluriformes Leiocassi longirostris,Silurus meridionalis,Pseudobagrus fulvidracoand Ictalurus punctatus were amplified with universal primers for ITS2 fragments,respectively.We constructed phylogenetic tree.The results indicated their genetic distance were: I.punctatus and P.fulvidraco(D=0.0661),P.fulvidraco and S.meridionalis(0.1100);S.meridionalis and L.longirostris(D=0.1184);I.punctatus and S.meridionalis(D=0.1266);P.fulvidraco and L.longirostris(D=0.1490);I.punctatus and L.longirostris(D=0.1503).The results showed that L.longirostris differentiated first,second was S.meridionalis and P.fulvidraco.I.punctatus is the latest.