姬松茸发酵液对小鼠抗运动疲劳及肝组织抗氧化能力的影响

研究姬松茸(Agaricus blazei)发酵液灌胃小鼠,对小鼠抗运动疲劳及肝组织抗氧化能力的影响.试验结果表明,与对照组(10 mg/kg·d ddH2O)相比,姬松茸发酵液中剂量组(20mg/kg·d)、高剂量组(30 mg/kg·d)小鼠的负重游泳、爬杆、耐缺氧时间均显著延长,血清尿素氮和血乳酸含量显著降低,肝糖原储存量显著升高,肝组织中SOD、GSH-Px活性显著提高,MDA值显著下降.说明灌胃姬松茸发酵液,能提高小鼠抗运动疲劳功能和加强肝组织抗氧化能力.

Anti-fatigue and Antioxidant Effects in Mice Administered Agaricus blazei Spent Culture Broth

Significant anti-fatigue and antioxidant effects were observed in mice lavaged with Agaricus blazei spent culture broth (SCB) remaining after harvesting fungal mycelium from 5-day old shaken (200 r/min),submerged cultures grown at 26 ℃ in 500 mL flasks containing 300 mL medium consisting of 2％ glucose,1％ yeast extract,1％ peptone,0.05％ MgSO4 and 0.05％ KH2 PO4) in distilled water and inoculated with 1％ (v/v) liquid spawn.Medium and high doses (20 and 30 mg/kg · d,respectively) of A.blazei spent culture broth (SCB) significantly prolonged the weight-load swimming time,rod climbing ability and hypoxia tolerance.Decreased levels of serum urea nitrogen,blood lactic acid,and malonaldehyde (MDA) in the liver homogenates,enhanced glutathione peroxidase (GSH-Px)and superoxide dismutase (SOD) activity in liver tissue,and increased liver glycogen levels,were also recorded in treated mice compared with a control group administered 10 mg/kg · d ddH2O.