3种ELISA法检测Bt杀虫蛋白的比较研究

运用3种ELISA法对提纯的Bt杀虫蛋白进行检测,结果表明:直接法ELISA和夹心法ELISA的灵敏度相同,都为0.94 ng/孔,但直接法ELISA中Bt蛋白量与OD值间呈极显著的线性关系,夹心法ELISA中Bt蛋白量与OD值间呈显著的线性关系;间接法ELISA的灵敏度较前两者低,使用HRP-IgG的灵敏度为15 ng/孔,Bt蛋白量与OD值间呈显著的线性关系,使用AKP-IgG的灵敏度为3.75ng/孔,Bt蛋白量与OD值间呈极显著的线性关系。

Comparison among Three ELISA Methods for Bt Toxin Protein

Three ELISA methods were experimented to assay purified Bt toxin protein. The rested result indicated that the minimal detectable value from both direct ELISA and double antibody sandwich ELISA was 0.94 ng/well. But there appeared very significant linear correlation between quantity of Bt toxin protein and their OD value in direct ELISA and there exited significant linear correlation between quantity of Bt toxin protein and in double antibody sandwich ELISA. The minimal detectable value by indirect ELISA was lower. When HRP-IgG was used, the minimal detectable value was 15 ng/well and there appeared significant linear correlation between quantity of Bt toxin protein and their OD value. When AKP-IgG was used, the minimal detectable value was 3.75ng/well and there appeared very significant linear correlation between quantity of Bt toxin protein and their OD value.