| 猪屎豆丛枝病植原体的分子检测与鉴定 |
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| 引用本文： | 李永,徐启聪,田国忠,郭民伟,朴春根.猪屎豆丛枝病植原体的分子检测与鉴定[J].林业科学,2010,46(1). |
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| 作者姓名： | 李永 徐启聪 田国忠 郭民伟 朴春根 |
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| 作者单位： | 中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室,北京,100091 |
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| 基金项目： | 国家科技基础条件平台建设项目(2005DKA21207);;国家自然科学基金项目(30471393)资助 |
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| 摘 要： | 植原体(phytoplasma),原称类菌原体(MLO),在植物和昆虫中广泛分布,为无细胞壁原核微生物,尚不能在人工培养基上离体培养.迄今,世界各地已统计有1000多种植物自然感染植原体病害(Seemüller et al.,1998),我国也报道了100多种植物植原体病害(赖帆等,2008).
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| 关 键 词： | 猪屎豆丛枝病 植原体 分子鉴定 序列测定 |
Molecular Identification of Crotalaria Witches'-Broom Phytoplasma |
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| Li Yong,Xu Qicong,Tian Guozhong,Guo Minwei,Piao Chungen.Molecular Identification of Crotalaria Witches'-Broom Phytoplasma[J].Scientia Silvae Sinicae,2010,46(1). |
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| Authors: | Li Yong Xu Qicong Tian Guozhong Guo Minwei Piao Chungen |
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| Institution: | Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest Ecology/a>;Environment and Protection/a>;Chinese Academy of Forestry Beijing 100091 |
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| Abstract: | Crotalaria witches'-broom phytoplasma (CrWB) collected from Hainan Province,was classified and identified by sequencing techniques and Virtual RFLP.Two different type universal primer pairs of phytoplasma were used to amplify a 1.8 kb fragment of 16S rDNA and a 1.3 kb fragment of rp gene.The PCR-amplified products were cloned and sequenced.The results of sequencing and homologuous comparison with other phytoplasmas showed that Crotalaria witches'-broom phytoplasma shared 99.9 % similarity with peanut witche... |
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| Keywords: | Crotalaria witches'-broom phytoplasma phytoplasma molecular identification sequencing |
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