Note: Occurrence and distribution ofCucumber mosaic virus infecting danshen (Salvia miltiorrhiza) in China |
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| Authors: | Z. M. Wu Y. Chen X. L. Xie C. X. Wen J. F. Chen M. G. Zhou |
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| Affiliation: | (1) National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China;(2) College of Plant Protection, Nanjing Agricultural University, 210095 Nanjing, China;(3) Institute of Economic Crop Research, Hebei Academy of Agricultural and Forestry Sciences, 050051 Shijiazhuang, China |
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| Abstract: | Cucumber mosaic virus (CMV) has resulted in much damage to Danshen (Salvia miltiorrhiza Bunge) crops in China. A 5-year survey was conducted in Hebei, Shandong, Sichuan, Shanxi, Henan and Gansu Provinces, all
major Danshen-growing areas. A total of 156 Danshen plant samples with CMV symptoms were collected and tested for the presence
of CMV by a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using polyclonal antibodies against CMV
and a positive control, according to the supplier’s instructions (Agdia). They were confirmed to be CMV by amplification of
complete coat protein gene and analysis of the gene sequence. The results showed that 122 samples were infected by CMV and
all of these CMV isolates belonged to subgroup IB.
http://www.phytoparasitica.org posting May 19, 2008.
Joint first authors. |
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| Keywords: | Cucumber mosaic virus
CMV distribution DAS-ELISA
Salvia miltiorrhiza |
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