俄引春小麦脂肪氧化酶活性基因的标记分析

为了改良黑龙江省小麦品种,加强其种质创新,利用与脂肪氧化酶基因(LOX)活性有关的2个遗传位点相连锁的3对分子标记,对247份俄罗斯引进的春小麦品种进行研究。结果表明:对于SSR标记Xwmc312,142份品种扩增出247bp特异条带,即Xwmc312-247基因型比例为57.49%,85份品种扩增出235bp特异条带,Xwmc312-235基因型比例为34.41%,20份品种扩增出227bp特异条带,Xwmc312-227基因型比例为8.10%。TaLOX-B1位点上,16份品种扩增出标记LOX16的特异条带,即高活性等位基因型TaLoxB1a分布比例是6.48%,230份品种扩增出标记LOX18特异条带,即低活性的等位基因型TaLOX-B1b分布比例是93.12%,有一个品种在此位点表现杂合。表明以TaLox-B1b基因型为主。2个位点不同等位基因组合共有6种,Xwmc312-247/TaLOX-B1b分布比例最高(53.85%),Xwmc312-22 7/TaLOX-B1a分布比例最低(0.41%),高活性组合Xwmc312-235/TaLOX-B1a(分布比例2.43%)等其它4种组合型介于二者之间,表明俄罗斯引进小麦品种以LOX低活性组合型为主。

Polymorphism of Lipoxygenase Genes in Russia Spring Wheat Cultivars

In order to improve the wheat varieties of Heilongjiang province, strengthen the germplasm innovation,247 spring wheat varieties from Russian were detected by 3 pairs of molecular markers linked with 2 lipoxygenase（LOX） loci. Tile results showed that for Qlpx. caas-lAL loci,there were 142 cuhivars amplified Xwmc312_z47 alleles with 247 bp specific bands,85 cultivars amplified Xwmc312-235 with 235 bp specific bands, 20 cuhivars amplified Xwmc312zz7 with 247 bp specific bands for SSR Xwmc312, distribution ratio of Xwmc31224z ,Xwmc312-235 and Xwmc312 -227 were 57.49% ,34.41%and 8.10% respectively. For TaLOX-B1 loci, 16 cuhivars amplified high-activity allele specific band （ TaLOX-Bla） for marker Loxl 6,230 eultivars am- plified low-activity allele specific band（TaLOX-Blb） for marker Lox18,1 cuhivar was hybrid,distribution ratio of TaLOX-Bla and TaLOX-Blb were 6.48% and 93.12% which suggested that TaLOX-Blb was the most in TaLOX-B 1 loci. There were six allelie combinations of in 2 loci, Xwmc312-247/TaLOX-Blb distribution ratio was the most （ 53.85 % ）, and that distribution ratio of Xwrnc312-225/TaLOX-B1a was least （0. 41%）. Other four allelic combinations were the medium. The data indicated that spring wheat varieties introduced from Rus- sian was based on the combination of low LOX activity.