| 冷冻液平衡时间和复温时间对小鼠胚胎冷冻解冻效果的影响 |
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| 引用本文： | 高建明,姜晓鹃,焦占海,焦革莉.冷冻液平衡时间和复温时间对小鼠胚胎冷冻解冻效果的影响[J].北京农学院学报,2000,15(3):27-30. |
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| 作者姓名： | 高建明 姜晓鹃 焦占海 焦革莉 |
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| 作者单位： | 北京农学院动物科学技术系,北京,102206 |
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| 摘 要： | 本试验以 1 5mol/L乙二醇为小鼠胚胎一步细管冷冻保护剂 ,观察小鼠胚胎在冷冻液中平衡时间和复温时间的冷冻解冻效果 .结果表明 ,以冷冻液中平衡 6 8～ 10 1min和细管解冻时在室温中停留 15s更利于胚胎在体外培养发育 ,其发育率为 86 96 %
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| 关 键 词： | 小鼠胚胎 平衡时间 复温时间 冷冻解冻 体外发育 |
The Effect of the Balance Times in the Freezing Liquid and Regainning Temperature Times on Freezing and Thawing Results in Mouse Embryos |
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| Gao Jianming,et al.The Effect of the Balance Times in the Freezing Liquid and Regainning Temperature Times on Freezing and Thawing Results in Mouse Embryos[J].Journal of Beijing Agricultural College,2000,15(3):27-30. |
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| Authors: | Gao Jianming |
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| Abstract: | 1.5mol/L
ethyleneglycol was used as one-step freezing protection liquid in mouse embryos. When the
mouse embryos were freezed and thawed, they were the balance 68～101 minutes in the freezing liquid and the stay 15 seconds
in the room. Their development rates were 86.96 in vitro. |
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| Keywords: | mouse epbryos balance times regainning times freezing and thawing development in vitro |
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