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| γ-氨基丁酸代谢旁路阻断对苏云金芽胞杆菌芽胞形成和晶体蛋白产量的影响 | |
| 引用本文： | 王维,高继国,朱莉,彭琦,束长龙,张杰,黄大昨方,宋福平.γ-氨基丁酸代谢旁路阻断对苏云金芽胞杆菌芽胞形成和晶体蛋白产量的影响[J].东北农业大学学报,2012(7):119-123. |
| 作者姓名： | 王维 高继国 朱莉 彭琦 束长龙 张杰 黄大昨方 宋福平 |
| 作者单位： | 1. 东北农业大学生命科学学院,哈尔滨150030 2. 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 3. 中国农业科学院生物技术研究所,北京100081 |
| 基金项目： | 国家973计划项目(2009CB118902);国家自然基金项目(31070083) |
| 摘 要： | γ-氨基丁酸旁路(GABA shunt)普遍存在于原核和真核生物体中,对于动物、植物和微生物具有不同的生理功能.苏云金芽胞杆菌gab基因簇中gabT和gabD基因所编码的γ-氨基丁酸转氨酶和琥珀酸半醛脱氢酶参与GABA shunt.在前期研究获得的苏云金芽胞杆菌HD-73菌株gabT、gabR(gab基因簇中的调控基因)和gabD基因缺失突变菌株的基础上,从菌株生长、芽胞产量和晶体蛋白产量等方面研究GABA shunt阻断对Bt芽胞和晶体形成的影响.结果表明,GABA shunt阻断对芽胞产量有一定影响,但对Bt生长和晶体蛋白产量无明显影响 |
| 关 键 词： | 苏云金芽胞杆菌 γ-氨基丁酸旁路 gab基因簇 晶体蛋白 |
Effect of GABA shunt disruption on sporulation and crystal protein production in Bacillus thuringiensis |
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| WANG Wei,GAO Jiguo,ZHU Li,PENG Qi,SHU Changlong,ZHANG Jie,HUANG Dafang,SONG Fuping.Effect of GABA shunt disruption on sporulation and crystal protein production in Bacillus thuringiensis[J].Journal of Northeast Agricultural University,2012(7):119-123. | |
| Authors: | WANG Wei GAO Jiguo ZHU Li PENG Qi SHU Changlong ZHANG Jie HUANG Dafang SONG Fuping |
| Institution: | 1.School of Life Sciences,Northeast Agricultural University,Harbin 150030,China;2.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;3.Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
| Abstract: | The GABA shunt is ubiquitous in most prokaryotic and eukaryotic organisms and it has distinct physiological functions in different organisms.The gab gene cluster which encodes GABA aminotransferase and succinate semialdehyde dehydrogenase involves in GABA shunt.In the study,the growth speed and the ability of sporulation and Cry protein production in gab gene cluster mutants and HD-73 wild-type strain were analyzed.The results showed that the ability of sporulation was reduced in all mutant strains.However,growth speed and the expression of crystal proteins had no difference between mutants and wild-type strain. |
| Keywords: | Bacillus thuringiensis GABA shunt gab gene cluster crystal protein |
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