Occurrence of Coxiella burnetii in Polish dairy cattle herds based on serological and PCR tests |
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| Institution: | 1. Department of Cattle and Sheep Diseases, National Veterinary Research Institute, 24-100, Puławy, Poland;2. Laboratory of Serological Diagnosis, National Veterinary Research Institute, 24-100, Puławy, Poland;1. Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran;2. Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran;3. Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;4. Department of Biostatistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran;5. Isfahan Veterinary Offices, Head of Public Health Supervision Section, Isfahan, Iran;6. Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran;7. Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran;1. Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Groennegaardsvej 8, DK-1870 Frederiksberg C, Denmark;2. Department of Epidemiology and Public Health, Faculty of Veterinary and Animal Science, Sylhet Agricultural University, Sylhet 3100, Bangladesh;3. Laboratory of Applied Statistics, Department of Mathematical Sciences, Faculty of Science, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen OE, Denmark;1. Aix-Marseille Univ, IRD, SSA, AP-HM, VITROME, IHU Méditerranée Infection, Marseille, France;2. School of Veterinary Sciences, El Harrach, Algeria;3. Aix-Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille, France;1. Department of Veterinary Medicine, University of Bari “Aldo Moro”, Bari, Italy;2. Lebanese University, Doctoral School of sciences and Technology, Beirut, Lebanon;3. Lebanese Agricultural Research Institute, Lebanon;4. Istituto Zooprofilattico Sperimentale della Sicilia \"A. Mirri\", Italy;5. Lebanese University, Faculty of Science, Beirut, Lebanon;6. Republic of Lebanon Ministry of Agriculture, Beirut, Lebanon;7. Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise “G. Caporale”, Teramo, Italy;8. Lebanese University, Faculty of Agricultural, Beirut, Lebanon;1. University of Lyon, VetAgroSup, Marcy L’Etoile, France;2. EPIA, UMR 0346, Epidemiologie des maladies animales et zoonotiques, INRA, VetAgroSup, 63122 Saint-Genès Champanelle, France;3. ANSES (French Agency for Food, Environmental and Occupational Health & Safety), Laboratory of Sophia Antipolis, Animal Q Fever Unit, Sophia Antipolis, France;4. IHAP, University of Toulouse, INRA, ENVT, Toulouse, France;5. ANSES, Animal Health Laboratory, EURL on Equine Diseases, Maisons-Alfort, France;6. CIRAD, UMR ASTRE, F-34398 Montpellier, France;7. LABÉO Frank Duncombe Laboratory, EA7450 BIOTARGEN, Université de Caen Normandie IFR 146 ICORE, 14053 Caen cedex 4, France;8. Aix-Marseille University, CNRS 7278, IRD 198, Inserm U1095, Assistance Publique-Hôpitaux de Marseille, URMITE, IHU Méditerranée-Infection, Marseille, France;1. Department of Clinical Sciences, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Pathobiology, Sanandaj Branch Islamic Azad University, Sanandaj, Iran |
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| Abstract: | The aim of the research was to assess the prevalence of antibodies to Coxiella burnetii in dairy cattle herds in Poland and to compare the results of real-time PCR and ELISA tests performed on bulk tank milk (BTM) samples. In total, 2635 serum samples collected from 969 dairy cattle herds from all provinces were tested using ELISA. Additionally, BTM specimens from 101 herds were analysed by ELISA and real-time PCR targeting IS1111 element. Presence of anti-C. burnetii antibodies was confirmed in 25.39% of serum samples in 237 herds (24.46%) and the herd-level seroprevalence in Voivodeships varied from 2.5% to 61.4%. Moreover, 46 (45.5%) of analysed bulk tank milk samples gave postive result in ELISA and microbial DNA was detected in 40 (39.6%) of tested herds. The comparative analysis of ELISA and real-time PCR results obtained for BTM samples using the chi-square test showed statistically significant relationship between results of both methods. |
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| Keywords: | Q fever Serology Real-time PCR Dairy cattle |
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