高温胁迫下坛紫菜中红藻糖苷及其异构体的含量变化

为了解热激应答下坛紫菜中红藻糖苷和异红藻糖苷含量的变化,以及两者与坛紫菜高温耐受性之间的联系,以ME-05坛紫菜为样品,采用高效液相色谱三重四级杆质谱联用技术(HPLC-MS)分析35℃高温胁迫和20℃恢复培养条件下,坛紫菜中红藻糖苷及其异构体含量的变化趋势,通过两者的含量波动,探讨其与坛紫菜耐受性之间的关系。结果表明,相同品种、相同日龄但生长地点不同的坛紫菜中,红藻糖苷含量稳定;不同生长阶段的坛紫菜经35℃高温胁迫后,红藻糖苷和异红藻糖苷含量减少,经恢复培养3 h后发现红藻糖苷和异红藻糖苷含量逐渐回升并超过对照组;红藻糖苷和异红藻糖苷的变化率为:Ⅲ组(生长期第75天)>Ⅳ组(生长期第135天)>Ⅱ组(生长期第45天)>Ⅰ组(丝状体),红藻糖苷的变化更为敏感且具有规律。综上,红藻糖苷与坛紫菜的生长状况和抗逆能力相关,可作为紫菜选育的指标物。本研究结果为进一步探讨坛紫菜在高温胁迫下的生理响应及健康养殖奠定了理论基础。

Different Variations of Floridoside and Isofloridoside in Pyropia haitanensis Under Heat Resistance

In order to investigate the variation of floridoside and isofloridoside of Pyropia haitanensis under high temperature environment, P. haitanensis ME-05 was used as experimental material to understand the relationship between the contents fluctuant and the high temperature resistance. High performance liquid chromatography coupled with mass spectrometry was used to analyze the variations of floridoside and isofloridoside of P. haitanensis under heat shock and recovery periods. The results showed that the contents of floridoside varied a little for P. haitanensis from different locations, but the same breed and age of P. haitanensis. When the heat shock temperature was set at 35℃, the contents of floridoside and isofloridoside were reduced by comparing to those of control group in all of P. haitanensis of different growth locations. When in the recovery period, the contents of them were gradually increased and exceeded the control group as the recovery time sustained to 3 h. The variation rates of floridoside and isofloridoside in different growth stages were shown as follows: Group Ⅲ(day 75 of growth)>Group Ⅳ(day 135 of growth)>Group Ⅱ(day 45 of growth)>Group Ⅰ(conchocelis). The variation of floridoside was more sensitive and regular than those of isofloridoside. It can be suggested that floridosides was related to growth and stress resistance of P. haitanensis, which can be used as a potential reference for the cultivation of P. haitanensis. The results of this study provide the basis for the further exploration of the physiological response under heat stress and healthy cultivation of P. haitanensis.