细胞分裂素对蝴蝶兰胚性愈伤组织诱导的影响

分别以6-Benzylaminopurine(6-BA)和2,4-Dichloro phenoxyacetic acid(2,4-D)诱导蝴蝶兰外植体.6-BA从嫩茎切块诱导出胚性愈伤组织,细胞富含淀粉粒,而2,4-D得到的愈伤组织不能分化.6-BA易于从嫩叶切片诱导胚性愈伤组织,进行体细胞胚发生过程的显微观察和代谢分析.6-BA诱导期间,DNA含量上升伴随胚性愈伤组织生长,水溶性蛋白变化稍延缓于RNA含量变化.培养第11天新出现12.13,13.08,66.30,97.25,104.31 ku蛋白质,可能与胚性细胞出现有关,第25天新出现40.87 ku蛋白质,与体细胞胚形成有关.

Effect of Cytokinin on Embryogenic Callus Induction in Phalaenopsis

Two types of explants, stem and leaf, from test tube seedlings of Phalaenopsis were cultured on the medium supplemented with 6-Benzylaminopurine(6-BA) and 2, 4 - dichloro phenoxyacetic acid(2,4-D) respectively. Embryogenic callus(EC), whose cells contained large amount of starch grains, was induced with 6-BA from the stem, whereas the callus induced with 2,4-D was unable to differentiate. The EC was also induced efficiently from leaf explant cultured on the medium supplemented with 6-BA. Histological observation and metabolic analysis were carried on during the somatic embryogenesis. When the explants were induced with 6-BA, the DNA content in the explants was increased with the growth of the EC. The RNA content changed earlier than the solubleprotein content. The SDS-PAGE profile of the soluble protein indicated that proteins(12.13 kDa,13.08 kDa, 66.30 kDa,97.25 kDa,104.31 kDa) came up at the 11th day of culture, which probably indicates the induction of embryo cells. And the protein of 40.87 kDa showed up at the 25th day of culture,indicating somatic embryogenesis.