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蓖麻不同部位蓖麻碱的含量及其杀虫活性
引用本文:刘进进,袁慧慧,王金威,潘铁英,蓝闽波.蓖麻不同部位蓖麻碱的含量及其杀虫活性[J].农药学学报,2013,15(2):239-242.
作者姓名:刘进进  袁慧慧  王金威  潘铁英  蓝闽波
作者单位:1.华东理工大学 上海市功能性材料化学重点实验室/分析测试中心,上海 200237
基金项目:上海市科技兴农重点攻关项目资助(沪农科攻字2010第2-1号).
摘    要:采用高效液相色谱(HPLC)外标法测定并比较了蓖麻Ricinus communis不同部位和不同生长期蓖麻叶中蓖麻碱的含量,并研究了其对甜菜夜蛾Spodoptera exigua的毒杀效果。结果表明,蓖麻不同部位中蓖麻碱的含量差异较大,由高到低依次为叶(1.005%)>籽壳(0.568%)>饼粕(0.451%)>茎(0.429%)>籽仁(0.235%);不同生长期蓖麻叶中蓖麻碱的含量也存在很大差别,其中鲜绿叶中的含量最高。提取的蓖麻碱对甜菜夜蛾具有良好的毒杀效果,施药72 h后对2龄幼虫的LC50值为0.35 mg/mL。

关 键 词:蓖麻    蓖麻碱    高效液相色谱    甜菜夜蛾    杀虫活性
收稿时间:2012/9/21 0:00:00
修稿时间:1/8/2013 12:00:00 AM

Ricinine content in different parts of Ricinus communis and its insecticidal activity
LIU Jinjin,YUAN Huihui,WANG Jinwei,PAN Tieying and LAN Minbo.Ricinine content in different parts of Ricinus communis and its insecticidal activity[J].Chinese Journal of Pesticide Science,2013,15(2):239-242.
Authors:LIU Jinjin  YUAN Huihui  WANG Jinwei  PAN Tieying and LAN Minbo
Institution:1.Shanghai Key Laboratory of Functional Materials Chemistry/Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, China
Abstract:Ricinine content in different parts of Ricinus communis and the leaves of different growth periods was determined and compared by using high performance liquid chromatography (HPLC) external standard method.The insecticidal activity of extracted ricinine towards Spodoptera exigua (Lepidoptera:Noctuidae) was investigated.The results showed that the content of ricinine varied in different parts of R.communis,and decreased in the order of leaf (1.005%)>seed shell (0.568%)>caster bean meal (0.451%)>stem (0.429%)>seed kernel (0.235%).Different contents of ricinine were also found in the leaves of various growth periods,and the highest content was detected in the fresh green leaves.The extracted ricinine exhibited insecticidal activity towards 2nd instar larvae of S.exigua,with LC50 value of 0.35 mg/mL after 72 h.
Keywords:Ricinus communis  ricinine  high performance liquid chromatography  Spodoptera exigua  insecticidal activity
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