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尾叶桉叶片脂肪酸成分的GC—MS分析
引用本文:张婧,谢耀坚,杜阿朋,李天会,吴志华.尾叶桉叶片脂肪酸成分的GC—MS分析[J].桉树科技,2011(1):9-13.
作者姓名:张婧  谢耀坚  杜阿朋  李天会  吴志华
作者单位:国家林业局桉树研究开发中心,广东湛江524022
基金项目:广东省自然科学基金项目(9145624536-4000003)、湛江市财政资金科技专项竞争性分配项目(20102030244)和“林业重大公益性行业科研专项”(201104003)
摘    要:以索氏提取和超声波提取两种方法对尾叶桉U6桉叶油进行了提取,并利用GC—MS对其成分进行了分析。结·果表明:桉叶油中共检出8种脂肪酸成分,其中饱和脂肪酸6种,分别为十四酸、棕榈酸、硬脂酸、花生酸、二十四烷酸和二十八酸,多不饱和脂肪酸2种:亚油酸和α-亚麻酸;两种方法中索氏法的提取率(7.69%)明显高于超声波法(6.51%),而所得不饱和脂肪酸组成相同,索氏提取物中不饱和脂肪酸α-亚麻酸为18.93%,亚油酸为7.72%;超声波提取物中α-亚麻酸为30.31%,亚油酸为6.48%。

关 键 词:尾叶桉  提取物  脂肪酸  多不饱和脂肪酸  气相色谱-质谱

Analysis of Fatty Acid Composition in the Leaves Oils of Eucalyptus urophylla by GC-MS
Institution:ZHANG Jing, XIE Yao - jian, DU A - peng, LI Tian - hui, WU Zhi - hua ( China Eucalypt Research Centre, Zhanjiang 524022, Guangdong, China )
Abstract:The extraction from Eucalyptus urophylla U6 leaves with Soxhlet and Ultrasonic methods were analyzed by GC - MS. The results showed that there were 8 kinds of fatty acids were detected, of which 6 kinds of saturated fatty acids: tetradecanoate, hexadeeanoic acid, octadecanoic acid, eicosanoic acid, tetracosanoic acid and octacosanoic acid, and two kinds of polyunsaturated fatty acids, (Z, Z, Z) - 9, 12, 15 - octadecatrienoic acid and (Z, Z) - 9, 12 - octadecadienoic acid. Extraction rate of Soxhlet method ( 7.69% ) was higher than ultrasonic method (6.51% ) significantly, and the main composition of polyunsaturated fatty acid extracted with two methods were the same, the relative content of (Z, Z, Z) - 9, 12, 15 - octadecatrienoic acid and (Z, Z) - 9,12 - ectadecadienoic acid were 18.93% and 7.72% in Soxhlet extraction respectively, and 30.31% and 6.48% in Ultrasonic extraction respectively.
Keywords:Eucalyptus urophylla  extraction  fatty acid  polyunsaturated fatty acid  GC - MS
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