Antisense nucleic acid of K-ras inhibits growth of lung adenocarcinoma cancer cell A549

AIM: To explore the mechanism of inhibiting lung adenocarcinoma cancer mediated by antisense nucleic acid of K-ras.METHODS: The expression of K-ras was detected in A549 cells and 6 lung adenocarinoma samples. The antisense expression vector of K-ras was successfully constucted (named antisense- K-ras-pcDNA3.1). After transfection, the growth curve and Apoptosis were determined by MTT assay and Annexin V staining, respectively. Cyclin A, cyclin D1, cyclin E, CDK2, CDK4, P53, Rb and caspase-3 were detected by Western blotting. RESULTS: K-ras was highly expressed in 4 samples of lung adenocarcinoma and A549 cells. In A549 cells transfected with antisense nucleic acid of K-ras, the cell growth was significantly inhibited and the apoptosis was visible.The expression levels of cyclin A, cyclin D1, cyclin E, CDK2 and CDK4 were significantly decreased, and the expression levels of P53, Rb and caspase-3 were significantly increased. CONCLUSION: The growth inhibition in A549 cells mediated by antisense nucleic acid of K-ras is related to the decreases in the expression of cyclin A, cyclin D1, cyclin E, CDK2 and CKD4 , and the increases in the expression of P53, Rb and caspase-3.