Effects of ginsenoside Rb1 on proteome from hippocampus of rats with epilepsy induced by Coriaria lactone

AIM: To explore the mechanism of development of Coriaria lactone (CL)-induced epilepsy and the neuroprotective effects of ginsenoside Rb1 (GRb1) on the process of epilepsy by identifying the proteins related to CL and GRb1 in rat hippocampus with two-dimensional electrophoresis (2-DE) technology.METHODS: The rat model of epilepsy was induced by CL. The rats in control group, CL epileptic group and GRb1 +CL epileptic group were decapitated and the hippocampus were collected. Two-dimensional electrophoresis was applied to separate the proteins from each group. Analysis of 2-DE maps was used to determine differential expression of proteins by ImageMaster 2D Platinum 5.0, and some differentially expressed protein spots were picked up for further identification by matrix-assisted laser sorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).RESULTS: Six proteins were successfully identified. Among these, the expression of brain creatine kinase, endophilin-A1 and UPF0628 protein C10orf96 homolog was lower in GRb1+CL epileptic group than those in CL epileptic group. The expression of cytochrome P-450, phosducin-like protein and bridging integrator 3 protein was lower in GRb1+CL epileptic group than those in control group.CONCLUSION: The protein expression profiles are significantly different among control group, CL epileptic group and GRb1+CL epileptic group. The identified proteins may be related to the neuroprotective effects of GRb1. Among these, brain creatine kinase, endophilin-A1 and UPF0628 protein C10orf96 homolog may be related to CL-induced seizures.