温度变化对家兔离体小肠平滑肌运动的影响

为了探究温度变化对动物消化道平滑肌运动的影响，本试验用生物信号处理系统测定了浴槽不同温度（39→32℃、32→39℃、39→42℃和42→39℃）对家兔离体小肠各段（十二指肠、空肠、回肠）平滑肌运动的影响。结果显示，当温度在39和32℃之间变化时家兔离体十二指肠和空肠运动的收缩频率同温度变化呈正相关，而十二指肠收缩幅度和收缩张力同温度变化呈负相关，但回肠仅表现在收缩频率同温度变化呈正相关；当温度从39℃升高到42℃时，十二指肠的收缩频率、收缩幅度和收缩张力变化与温度在39和32℃之间变化情况相同，但是空肠和回肠的规律性较差；当温度从42℃高温降低到39℃时，3段离体小肠运动不能恢复到39℃时的运动状态，家兔小肠离体试验浴槽最适温度为37℃。本研究结果提示，在进行动物小肠离体试验时应注意浴槽温度对小肠运动的影响，同时本研究结果也为探究由于环境温度变化导致的动物胃肠运动异常的机理提供参考。

Effects of Temperature Changes on the Contractility of Isolated Small Intestinal Smooth Muscle in Rabbits

In order to explore the influence of temperature changes on animal digestive tract smooth muscle,the contractility of isolated rabbit small intestine (duodenum,jejunum and ileum) were measured at different temperatures (39→32℃,32→39℃,39→42℃ and 42→39℃) using the biological signal processing system.The results showed that the contraction frequency of duodenum and jejunum was positively correlated with temperature changes between 39 and 32℃,while the amplitude and tension of duodenum were negatively correlated with temperature changes.Only the contraction frequency of ileum was positively correlated with temperature changes between 39 and 32℃.Changes of frequency,amplitude and tension of duodenum between 39 and 42℃ were similar with those changes between 39 and 32℃.However,the contraction of jejunum and ileum was irregular.When the temperature was decreased from 42 to 39℃,the contraction of small intestine could not return to the condition in 39℃.The results suggested that the optimal bath temperature was 37℃ in contraction test of rabbit small intestine in vitro,and the present results could be referenced to explore the disorder of gastrointestinal movement due to the change of environment temperature in animals.