荧光标记微卫星分析广西树鼩群体的遗传多态性

选取10个微卫星位点,结合荧光标记(FAM) PCR扩增技术,运用毛细管电泳检测扩增产物,利用Microsatellite Toolkit、PopGene软件计算遗传相关指标,探讨广西树鼩群体的遗传多态性。结果发现,在10个微卫星基因座中,共检测到64个等位基因,平均等位基因数为6.4个,平均有效等位基因数为3.7892,平均观察杂合度和平均期望杂合度为0.6156和0.6963,平均多态信息含量为0.6367,其中7个是高度多态位点,3个属中度多态位点。此外,在10个微卫星基因座中,有5个位点偏离Hardy-Weinberg平衡(P<0.01)。结果表明广西树鼩遗传多态性较丰富,所选微卫星位点基本可用于评估广西树鼩群体遗传多样性。

The Genetic Polymorphism Analysis in Tree Shrew(T.belangeri chinensis) based on Fluorescent Microsatellite

A total of 10 fluorescent labeling microsatellite markers detected by capillary electrophoresis were selected to analyze the genetic diversity of tree shrew(T.belangeri chinensis).Microsatellite Toolkit and PopGene softwares were used to calculate the genetic correlation indexes to assess the genetic diversity of the population.64 alleles with 6.4 alleles per locus were identified among 10 microsatellite loci.The average effective number of alleles, observed heterozygosity, expected heterozygosity and polymorphic information content were 3.7892, 0.6156, 0.6963, and 0.6367, respectively.Seven of all loci were high polymorphic, and three were moderate polymorphic.Five loci extremely significantly deviated from Hardy-Weinberg equilibrium(P<0.01).These novel polymorphic microsatellite markers indicated high genetic diversity, which will be used to evaluate genetic diversity in tree shrew.