断奶应激对奶水牛犊牛血液生理生化指标和免疫功能的影响

为研究断奶应激对奶水牛犊牛的影响，选用5头健康犊牛，分别于断奶前1 d、断奶后1、14和21 d进行采血，测定犊牛的血常规、血清生化指标和免疫指标。结果显示，奶水牛犊牛的白细胞数、淋巴细胞数、红细胞数、单核细胞数、红细胞压积、天冬氨酸转氨酶活性、血糖含量、皮质醇含量、免疫球蛋白G含量在断奶前后无显著变化（P > 0.05）；中性粒细胞数在断奶后21 d显著低于断奶前1 d（P < 0.05）；血清乳酸脱氢酶、丙氨酸转氨酶活性在断奶后显著高于断奶前（P < 0.05）；血清尿素氮含量在断奶后21 d显著高于断奶前1 d（P < 0.05）；血红蛋白含量在断奶21 d显著低于断奶前1 d（P < 0.05）。结果表明断奶应激不仅影响了奶水牛犊牛的生理机能，而且降低了其免疫力和抵抗力。

Effect of Weaning Stress on Blood Physiology and Biochemistry Indexes and Immune Function in Dairy Buffalo Calves

To study the effect of weaning stress on dairy buffalo calves,5 healthy calves were chosen and weaned,and the blood samples were collected at 1 day before weaning,and 1,14 and 21 days after weaning to analysis blood physiology and biochemistry indexes and immune function.The results showed that the count of white blood cell,lymphocyte,erythrocyte,monocytes and hematocrit,aspartate aminotransferase activity,blood glucose,cortisol and immunoglobulin G contents of dairy buffalo calves were no significant difference between before and after weaning treatments (P > 0.05).The count of neutrophils at 21 d after weaning were significantly lower than that of 1 d before weaning of dairy buffalo calves (P < 0.05).However,lactate dehydrogenase and alanine aminotransferase activity in weaned treatments were significantly higher than those of animals in the treatment before weaning (P < 0.05).The serum urea nitrogen of dairy buffalo calves at 21 d after weaning was significantly higher than that of 1 d before weaning (P < 0.05) and hemoglobin content at 21 d after weaning was significantly lower than that at 1 day before weaning (P < 0.05).It indicated that the physiological and immunity function of dairy buffalo calves were challenged under weaning stress.