H1N1亚型流感病毒鸡胚增殖参数的优化

为了研究最佳的H1N1亚型流感病毒鸡胚增殖参数,本试验进行了孵化前种蛋的选择与保存、鸡胚孵化中各参数设定等因素对H1N1亚型流感病毒产毒量影响的研究。其中种蛋的选择与保存,主要考察了蛋重、蛋形指数、保存期、消毒时间及方法等因素,结果显示,蛋重为55~65 g,蛋形指数为1.30~1.35,种蛋保存期为1~4 d,保存温度为16~18 ℃,保存湿度为70%~80%,保存期种蛋的甲醛熏蒸消毒时间为30 min时,可以为H1N1亚型流感疫苗生产提供最佳的种蛋。孵化过程中孵化参数对H1N1亚型流感病毒产毒量的影响,本试验主要将孵化温度、湿度、翻蛋、通风等参数作为研究对象,结果显示在生产H1N1亚型流感疫苗时,最佳孵化参数设定为:温度1~7 d为38.2 ℃、8~9 d为38.0 ℃、10 d为37.8 ℃,湿度1~10 d为65%~70%,翻蛋频率为1次/2 h,前后倾角各为45°,通风风门设定为1~5 d为4、6~10 d为5。本试验结果为H1N1亚型流感疫苗生产提供优质的鸡胚孵化技术,确保鸡胚尿囊液的质量和收获量。

Optimization of Proliferation Parameter of H1N1 Influenza Virus in Chicken Embryo

In order to study the best propagation parameters of H1N1 subtype influenza virus in chicken embryo,this study was conducted to research the effects of egg selection and preservation before hatching and hatching parameters setting on production of H1N1 subtype influenza virus.In the egg selection and preservation,we mainly investigated the egg weight,egg shape index,storage period,disinfection time and method and other factors,the results showed that egg weight of 55 to 65 g,egg shape index of 1.30 to 1.35,egg storage period of 1 to 4 d,storage temperature of 16 to 18 ℃,humidity of 70% to 80%,during storage period,eggs formaldehyde fumigation time 30 min,we could provide the best eggs for H1N1 subtype influenza vaccine production.The effect of hatching parameters on production of H1N1 subtype influenza virus,this experiment mainly investigaed incubation temperature,humidity,times of turning over eggs,aeration and other parameters,the results showed that in the production of H1N1 subtype influenza virus,the optimal parameter set to hatch:temperature 1 to 7 d was 38.2 ℃,8 to 9 d was 38.0 ℃,10 d was 37.8 ℃;Humidity was 65% to 70% from 1 to 10 d;Turning over eggs frequency was 1 times/2 h,before and after each dip 45°;1 to 5 d aeration set to 4,6 to 10 d set to 5.The results provided quality chicken hatchery technology for H1N1 subtype influenza vaccine production in order to ensure the quality and yield of allantoic fluid.