| 黄瓜无毛基因gl-2的遗传分析和定位 |
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| 引用本文： | 杨双娟,苗晗,张圣平,程周超,周健,董邵云,Todd C．Wehner,顾兴芳.黄瓜无毛基因gl-2的遗传分析和定位[J].园艺学报,2011,38(9):1685-1692. |
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| 作者姓名： | 杨双娟 苗晗 张圣平 程周超 周健 董邵云 Todd C．Wehner 顾兴芳 |
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| 作者单位： | 1. 中国农业科学院蔬菜花卉研究所,北京,100081
2. 美国北卡罗来纳州立大学园艺系,NC 27695,USA |
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| 基金项目： | 国家现代农业产业技术体系专项资金资助项目(CARS-25); 农业部园艺作物生物学与种质创制重点实验室资助项目 |
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| 摘 要： | 以黄瓜（Cucumis sativus L.）有毛类型‘9110Gt’（P1）和无毛突变体‘NCG-042’（P2）为试材，对无毛基因gl-2进行遗传分析和基因定位研究。结果表明，黄瓜的有毛、无毛性状由一对核基因控制，有毛对无毛为显性。结合分离群体分组混合分析法（bulked segregant analysis，BSA），以F2为作图群体，筛选得到18对与黄瓜无毛基因gl-2相关的SSR引物，构建了gl-2基因的SSR连锁群，并将该基因定位在黄瓜第2染色体上，两侧最近的连锁标记为SSR10522和SSR13275，遗传距离分别为0.6 cM和3.8 cM。经过回交群体验证，SSR10522和SSR13275的正确率分别为94.4%和91.6%。
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| 关 键 词： | 黄瓜 无毛基因gl-2 遗传分析 基因定位 SSR |
| 收稿时间： | 2011-6-16 |
| 修稿时间： | 2011-8-23 |
Genetic Analysis and Mapping of gl-2 Gene in Cucumber(Cucumis sativus L.) |
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| YANG Shuang-juan,MIAO Han,ZHANG Sheng-ping,CHENG Zhou-chao,ZHOU Jian,DONG Shao-yun,Todd C.Wehner, GU Xing-fang.Genetic Analysis and Mapping of gl-2 Gene in Cucumber(Cucumis sativus L.)[J].Acta Horticulturae Sinica,2011,38(9):1685-1692. |
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| Authors: | YANG Shuang-juan MIAO Han ZHANG Sheng-ping CHENG Zhou-chao ZHOU Jian DONG Shao-yun Todd CWehner GU Xing-fang |
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| Institution: | YANG Shuang-juan~1,MIAO Han~1,*,ZHANG Sheng-ping~1,CHENG Zhou-chao~1,ZHOU Jian~1,DONG Shao-yun~1,Todd C.Wehner~2,and GU Xing-fang~1,**(1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China,2Department of Horticultural Science,North Carolina State University,Raleigh,NC 27695-7609,USA) |
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| Abstract: | Genetic analysis and gene mapping were carried out on gl-2 gene in cucumber(Cucumis sativus L.)using 9110G(twith trichomes)and NCG-042(glabrous mutant)as experimental materials.The genetic analysis showed having trichomes or not is determined by a single nuclear gene,and the trait of having trichomes(Gl-2)is dominant to the glabrous(gl-2)in cucumber.Bulked segregant analysis(BSA) and simple sequence repeat(SSR)techonology were employed to mapping gl-2 gene of cucmber in F2 population.gl-2 gene was mapped to... |
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| Keywords: | cucumber gl-2 gene genetic analysis gene mapping SSR marker |
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