| 梅单瓣复瓣花的相关分子标记初探 |
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| 引用本文： | 高志红,韩振海,章镇,张玉明.梅单瓣复瓣花的相关分子标记初探[J].园艺学报,2003,30(5):612-614. |
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| 作者姓名： | 高志红 韩振海 章镇 张玉明 |
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| 作者单位： | (中国农业大学园艺植物研究所, 北京100094 ;南京农业大学园艺学院, 南京210095) |
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| 基金项目： | 国家自然科学基金项目(3 0 0 70 53 0 )，国际基金资助项目(D/32 3 2 -1) |
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| 摘 要： | 应用RAPD分子标记技术和SCAR 分子标记技术研究了梅单瓣花与复瓣花相关的分子标记。结果表明从34 个随机引物中筛选到一个多态性引物OPP01 , 扩增出只有单瓣花类型具有的分子量为1899 bp的DNA 特异片段, 即OPP01-DS-1899 ; 根据该序列设计大小分别为25 bp 和24bp 的一对引物SSD-1 和SSD-2 ,将RAPD 标记转为SCAR 标记, 该标记大小为1079 bp 。
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| 关 键 词： | 梅 复瓣 单瓣 分子标记 |
| 文章编号： | 0513-353X(2003)05-0612-03 |
| 收稿时间： | 2002-11-29 |
| 修稿时间： | 2002年11月29 |
Identification of RAPD and SCAR Markers Linked to the Single-double Flower Gene in Japanese Apricot(Prunus mume Sieb.et Zucc.) |
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| Gao Zhihong ,Han Zhenhai ,Zhang Zhen ,and Zhang Yuming.Identification of RAPD and SCAR Markers Linked to the Single-double Flower Gene in Japanese Apricot(Prunus mume Sieb.et Zucc.)[J].Acta Horticulturae Sinica,2003,30(5):612-614. |
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| Authors: | Gao Zhihong Han Zhenhai Zhang Zhen and Zhang Yuming |
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| Institution: | 1. Department of Thoracic Surgery, China-Japan Union Hospital, University, Changchun Jilin, 130031, China;
2. Department of Ophthalmology, The Second Clinical Hospital, University, Changchun Jilin 130041, China |
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| Abstract: | Randomly amplified polymorphic DNA(RAPD)and sequence characterized amplified reg ion(SCAR)marker were performed to detect markers linked to the single double flo wer in Japanese apricot(Prunus mume Sieb.et Zucc.).Primer OPP01 was identif ied from 34 10-mer primers examined.The fragment was cloned and sequenced as 18 99 base pairs.According to the sequence,one primer pairs were designed to conver t the RAPD marker to SCAR marker.The length of the amplified fragment of SCAR ma rker was 1079 base pairs. |
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| Keywords: | Japanese apricot Double flower Single flower Molec ular marker |
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