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| 茶树培养细胞花色苷累积型与非累积型株系间的差异性研究 | |
| 引用本文： | 成浩,李素芳,孙锦荷.茶树培养细胞花色苷累积型与非累积型株系间的差异性研究[J].茶叶科学,2000,20(2):124-128. |
| 作者姓名： | 成浩 李素芳 孙锦荷 |
| 作者单位： | 1. 浙江大学原子核农业科学研究所,浙江杭州,310029;中国农业科学院茶叶研究所,浙江杭州,310008 2. 浙江大学原子核农业科学研究所,浙江杭州,310029 3. 中国农业科学院茶叶研究所,浙江杭州,310008 |
| 基金项目： | 国家自然科学基金! (39770 476 )，中国农业科学院院长基金! (96 2 31 4 )资助项目 |
| 摘 要： | 从细胞生长、色素和儿茶素等次生产物组成与含量、苯丙氨酸解氨酶活性以及产物合成动态分析等几方面 ,研究比较了积累色素与不积累色素的两类茶树培养细胞系之间的差异。发现积累色素的细胞系中 ,儿茶素与原花色素的形成能力也得到了大幅度提高 ,且与苯丙氨酸解氨酶活性无关。这一结果表明 ,茶树高产细胞系筛选中 ,可采用细胞色泽为指标 ,使筛选工作得以简化。 |
| 关 键 词： | 茶树 细胞培养 细胞系 花色苷 儿茶素 |
Differences between Anthocyanin Accumulating and Non-accumulating Subclones of Tea Cultured Cells |
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| CHENG Hao,LI Su-fang,SUN Jin-he.Differences between Anthocyanin Accumulating and Non-accumulating Subclones of Tea Cultured Cells[J].Journal of Tea Science,2000,20(2):124-128. | |
| Authors: | CHENG Hao LI Su-fang SUN Jin-he |
| Institution: | CHENG Hao 1,2,LI Su fang 1,SUN Jin he 2 [WT6BX] |
| Abstract: | The diferrences in cell growth, composition and levels of anthocyanins and catechins, activity of phenylalanine ammonia lyase and dynamics of production between anthocyanin accumulating and non accumulating subclones of tea cultured cells were examined It was found that the levels of catechins and proanthocyanins in those subclones accumulating anthocyanins were also raised, and were independent of the activity of phenylalanine ammonia lyase The results implied that the color of cells could be used as indicator in the selection of high yielding cell clones |
| Keywords: | Tea Cell culture Subclone Anthocyanins Catechins |
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