| 利用SRAP标记绘制中国芥菜基因源分子指纹图谱 |
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| 引用本文： | 王玉红,金关荣,陶爱芬,祁建民,张杨文,彭琼,沈焕明.利用SRAP标记绘制中国芥菜基因源分子指纹图谱[J].热带作物学报,2016,37(9):1719-1724. |
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| 作者姓名： | 王玉红 金关荣 陶爱芬 祁建民 张杨文 彭琼 沈焕明 |
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| 作者单位： | 1. 作物遗传育种与综合利用教育部重点实验室 福建省分子设计育种实验室,福建农林大学 福建福州,350002;2. 浙江省萧山棉麻研究所,浙江萧山,311202 |
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| 基金项目： | 福建省科技厅对外合作项目：中贝粮油及特色经济作物育种与新品种示范推广项目（No. 2015i0001）；福建省高水平大学建设项目（No. 612014005）。 |
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| 摘 要： | 以中国9个省份和地区的86份芥菜种质资源为供试材料,利用SRAP分子标记和DNA指纹图谱分析软件,绘制芥菜遗传资源基因组DNA指纹图谱。从270对SRAP引物中筛选出40对多态性明显、条带清晰的引物组合,对86份供试材料基因组DNA分子进行扩增,共扩增出632条谱带,其中多态性条带427条,多态性比率为67.56%,表明芥菜种质资源遗传多样性较丰富。以供试的86份芥菜种质资源的SRAP扩增条带为基础,建立供试材料扩增条带指纹数据库的Excel文件,然后利用自主开发的DNA指纹图谱软件,构建86份芥菜种质资源的DNA指纹图谱,获得了所有芥菜种质资源的分子"身份证"。结果表明,SRAP分子标记非常适合芥菜DNA指纹图谱的构建。
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| 关 键 词： | 芥菜 种质资源 DNA指纹图谱 SRAP标记 |
Construction of Molecular Fingerprinting Map of Leaf Mustard[Brassica juncea(L.)Czern et Coss]
in China with SRAP Markers |
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| WANG Yuhong,JIN Guanrong,TAO Aifen,QI Jianmin,ZHANG Yangwen,PENG Qiong and SHEN Huanming.Construction of Molecular Fingerprinting Map of Leaf Mustard[Brassica juncea(L.)Czern et Coss]
in China with SRAP Markers[J].Chinese Journal of Tropical Crops,2016,37(9):1719-1724. |
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| Authors: | WANG Yuhong JIN Guanrong TAO Aifen QI Jianmin ZHANG Yangwen PENG Qiong and SHEN Huanming |
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| Institution: | Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education / Fujian Provincial Key Laboratory of Crop Molecular Design Breeding, Fujian Agriculture and Forestry University;Xiaoshan Institute of Cotton and Fiber Crop Science;Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education / Fujian Provincial Key Laboratory of Crop Molecular Design Breeding, Fujian Agriculture and Forestry University;Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education / Fujian Provincial Key Laboratory of Crop Molecular Design Breeding, Fujian Agriculture and Forestry University;Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education / Fujian Provincial Key Laboratory of Crop Molecular Design Breeding, Fujian Agriculture and Forestry University;Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education / Fujian Provincial Key Laboratory of Crop Molecular Design Breeding, Fujian Agriculture and Forestry University;Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education / Fujian Provincial Key Laboratory of Crop Molecular Design Breeding, Fujian Agriculture and Forestry University |
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| Abstract: | |
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| Keywords: | Mustard Germplasm DNA fingerprint SRAP marker |
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