不同贮藏方式对4种桉树花粉活力影响的研究

目的]研究不同贮藏方式对4种桉树花粉活力的影响,探索出适宜桉树花粉贮藏的低温条件和贮藏方式。方法]以尾叶桉、尾叶桉×巨桉、巨桉和邓恩桉花粉为试验材料,对其进行常温、低温、低温冷冻和超低温冷冻贮藏,并对不同贮藏时间内的花粉活力进行检测。结果]适合桉树花粉萌发的最佳培养基为20%蔗糖+6%琼脂+0.1‰硼酸;贮藏方式和贮藏时间对花粉活力有显著影响,常温真空贮藏,尾叶桉和巨桉花粉贮藏30d后,萌发率降至10%以下,尾叶桉×巨桉和邓恩桉花粉贮藏50d后,花粉萌发率降至10%以下,低温冷冻和超低温冷冻贮藏1年的桉树花粉萌发率仍保持在35%以上;不同的解冻方式对花粉萌发率有显著影响,以38℃水浴3min化冻效果最好。结论]实际工作中选用低温冷冻贮藏花粉,可以得到既经济又高效的收益。

Study on Effects of Different Storage Methods on Pollen Viability of 4 Eucalyptus

Objective] The aim was to research the effects of different storageMethods on pollen viability of 4 eucalyptus, and explore the suitable low temperature condition and storageMethodfor pollen 9torage of eucalyptus. Method] Taking the pollens of E. urophylla S. T. Blake, E. urophylla S. T. Blake × E. grandis W. Hill exmaiden, E. grandis W. Hill exmaiden and E. dunnii Maiden as test material, the pollens were preserved in room temperature, low temperature, low temperature freezing and ultra low temperature freezing. The pollen viabilities during different storage time were determined. Result] The best medium for eucalyptus pollen germination was 20% sucrose + 6% agar + 0. 1 ‰ boric acid. StorageMethods and time had significant effect on pollen viability. After 30 days, the pollen germination rate of E. urophylla and E. dunnii declined to 10% , after 50 days, the pollen germination rate of E. urophylla S. T. Blake × E. grandis W. Hill exmaiden and E. dunnii Maiden declined to 10% in room temperature. The pollen was preserved in low temperature freezing and ultra low temperature freezing for one years, the pollen germination rate was still more than 35%. Pollen germination rate was also affected by different thawingMethods, and 38 ℃ water bath for 3 minutes was the bestMethod. Conclusion] Selecting low temperature freezingMethodto preserve pollen in practical work, it can obtain economic and efficient benefit.