| 同时高产甘露聚糖肽与乳酶生发酵条件的研究 |
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| 引用本文： | 冯锁民,任淑娟,杨颖,王黎,惠乐妮.同时高产甘露聚糖肽与乳酶生发酵条件的研究[J].安徽农业科学,2011(19):11889-11891. |
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| 作者姓名： | 冯锁民 任淑娟 杨颖 王黎 惠乐妮 |
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| 作者单位： | 西安医学院;陕西省人民医院药剂科;陕西康惠制药股份有限公司; |
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| 基金项目： | 2008年西安医学院博士科研启动金项目(2008DOC3); 2010年陕西省卫生厅科研基金项目(2010D03) |
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| 摘 要： | 目的]探索同时高产甘露聚糖肽和乳酶生的最佳发酵条件。方法]以甲型溶血性链球菌H1S-33号为菌株,进行液体发酵培养,在各个时间段分别取样测定活菌数及甘露聚糖肽含量,确定最佳培养条件。结果]在液体发酵过程中,28h发酵液中甘露聚糖肽含量最高为69.2μg/ml,活菌数最多为2.38×1016个/ml。结论]同时高产甘露聚糖肽与乳酶生发酵条件为：培养基由牛肉膏、蛋白胨、酵母膏、氯化钠组成,发酵温度37℃,最佳发酵时间28h。
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| 关 键 词： | 甘露聚糖肽 乳酶生 甲型溶血性链球菌 J＋--2酵 培养基 细菌计数 |
Research for the Fermentation Condition of High Yield Mannatide and Lactasin Simultaneously |
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| FENG Suo-min et al.Research for the Fermentation Condition of High Yield Mannatide and Lactasin Simultaneously[J].Journal of Anhui Agricultural Sciences,2011(19):11889-11891. |
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| Authors: | FENG Suo-min |
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| Institution: | FENG Suo-min et al(Xi'an Medical University,Xi'an,Shaanxi 710021) |
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| Abstract: | Objective]The aim was to explore the fermentation condition of high yield mannatide and lactasin simultaneously.Method]In order to obtain the best culture conditions,in the period of liquid fermentation culture,using α-hemalytic streptococci 33 as strains,all living bacteria number and mannatide content were determined respectively.Result]In the liquid fermentation process,the highest content of mannatide was 69.2 μg/ml,the maximum number of living bacteria was 2.38 × 1016 /ml.Conclusion] The best fermentation conditions were made from beef cream medium,peptone,yeast creams,sodium chloride composition,at temperature 37 ℃ with 28 h. |
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| Keywords: | Mannatide Lactasin α-hemalytic streptococci 33 Fermentation Medium Bacteria count |
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