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| 巢式PCR检测H1亚型禽流感病毒方法的建立 | |
| 引用本文： | 郭捷,谢芝勋,罗思思,刘加波,邓显文,谢志勤,庞耀珊,范晴.巢式PCR检测H1亚型禽流感病毒方法的建立[J].中国畜牧兽医,2013,40(9):23-26. |
| 作者姓名： | 郭捷 谢芝勋 罗思思 刘加波 邓显文 谢志勤 庞耀珊 范晴 |
| 作者单位： | 1. 广西大学, 动物科学技术学院, 广西南宁 530004; 2. 广西壮族自治区兽医研究所, 广西畜禽疫苗新技术重点实验室, 广西南宁 530001 |
| 基金项目： | 广西科技项目(桂科攻10100014-5和桂科专项11-3);广西特聘专家专项(2011B020);广西科技攻关重大专项(桂科重1222003-2-4);广西自然科学基金(2011GXNSFA018088)。 |
| 摘 要： | 为建立快速、准确检测H1亚型禽流感病毒(avian influenza virus,AIV)的方法,本研究针对H1亚型AIV血凝素(HA)基因保守区设计合成了2对特异性引物并优化反应条件进行巢式PCR反应。特异性和敏感性试验结果表明,该PCR方法能特异性检测到H1亚型AIV,其敏感性最低能检测到13.2 fg/μL的H1亚型AIV RNA模板。本研究建立的巢式PCR方法为H1亚型AIV的早期诊断及有效防制提供了快速、特异且敏感的检测方法。 |
| 关 键 词： | H1亚型禽流感病毒 巢式PCR 检测 |
| 收稿时间： | 2013-01-23 |
Establishment of a Nested PCR Technique for Detection of H1 Subtype Avian Influenza Virus |
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| GUO Jie,XIE Zhi-xun,LUO Si-si,LIU Jia-bo,DENG Xian-wen,XIE Zhi-qin,PANG Yao-shan,FAN Qing.Establishment of a Nested PCR Technique for Detection of H1 Subtype Avian Influenza Virus[J].China Animal Husbandry & Veterinary Medicine,2013,40(9):23-26. | |
| Authors: | GUO Jie XIE Zhi-xun LUO Si-si LIU Jia-bo DENG Xian-wen XIE Zhi-qin PANG Yao-shan FAN Qing |
| Institution: | 1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China; 2. Guangxi Key Laboratory of Animal Vaccines and New Technology, Guangxi Veterinary Research Institute, Nanning 530001, China |
| Abstract: | In order to develop a simple and quick method to detect H1 subtype avian influenza virus (AIV),two pairs of primers were designed according to the sequences of HA gene of H1 subtype AIV, and a nested polymerase chain reaction (nPCR) method was developed for detection of H1 subtype AIV. The specificity and sensitivity test showed that this nPCR was only specific to the H1 subtype AIV and could detect 13.2 fg/μL of AIV RNA. The results demonstrated that the optimized nPCR assay was rapid, specific and sensitive for detection of H1 subtype AIV. |
| Keywords: | H1 subtype avian influenza virus nested PCR detection |
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