Molecular Identification and Genetic Evolution Analysis of Haemophilus parasuis 16S rRNA Gene in Local Pig Origin |
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Authors: | CHANG Hong-tao LIU Hui-min DU Ji-mei CHEN Lu ZHAO Jun WANG Xin-wei YAO Hui-xia YANG Xia LI Hong-yan WANG Chuan-qing |
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Institution: | 1. College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China;2. Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China |
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Abstract: | To study the molecular genetic evolution characteristics of Haemophilus parasuis(Hps), PCR was used to determine the 16S rRNA gene of the strain XY0501 isolated from local pigs in Henan province, and genetic evolution analysis was conducted. The results showed that the amplication of 16S rRNA of the isolate XY0501 was 822 bp, the nucleotide sequence similarity among the reference strains was 97.1% to 99.3%, and 99.3% with the strain serotype 5. The phylogenetic tree based on 16S rRNA revealed that the Hps isolate XY0501 from local pigs belonged to the same branch with reference strain serotype 5. The results identified that the Hps infection could cause severe clinical symptoms in pigs, but infection source need to be further investigation,in addition, 16S rRNA of Hps of different serotypes was conserved and no species difference. |
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Keywords: | Haemophilus parasuis strain from local pig PCR 16S rRNA molecular genetic evolution |
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