| 绵羊痘病毒E3L蛋白N端结构和功能预测 |
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| 引用本文： | 卢昌,吴国华,颜新敏,赵志荀,于少雄,叶奕优,朱海霞,李健,张强. 绵羊痘病毒E3L蛋白N端结构和功能预测[J]. 中国畜牧兽医, 2013, 40(6): 85-89 |
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| 作者姓名： | 卢昌 吴国华 颜新敏 赵志荀 于少雄 叶奕优 朱海霞 李健 张强 |
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| 作者单位： | 1. 中国农业科学院兰州兽医研究所, 家畜疾病病原生物学国家重点实验室, 甘肃兰州 730046;2. 深圳出入境检验检疫局动植物检验检疫技术中心, 广东深圳 518045 |
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| 基金项目： | 国家自然科学基金项目(31001056、31101802、31201892);国家"十二五"高技术研究发展计划(863)项目(2012AA101304);甘肃省自然科学基金项目(1208RJZA101)。 |
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| 摘 要： | E3L蛋白是痘病毒的一类早期表达的非结构蛋白,其主要抑制宿主的先天性免疫,与病毒的致病力和宿主嗜性有关,但是目前对绵羊痘病毒E3L蛋白的研究较少。作者运用生物信息学的方法,用已知的E3L蛋白N端结构作为模板,并使用WebLab、SWISS-MODEL和ExPASY等在线软件预测其功能结构域和二、三级结构及氨基酸组成,对其作为Zα潜在家族成员的真实性、可靠性作出理论上的判断。分析结果表明,绵羊痘病毒E3L蛋白N端结构符合Zα家族蛋白的一些基本的结构特征,具有其他E3L蛋白N端相同的功能结构,为该蛋白质N端结构属于Zα蛋白家族提供了一些理论依据。
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| 关 键 词： | 绵羊痘病毒 E3L蛋白 Z-DNA 生物信息学 |
| 收稿时间： | 2012-11-16 |
Function Prediction and Structure Analysis of N-terminal of E3L Protein in Sheeppoxvirus |
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| LU Chang,WU Guo-hua,YAN Xin-min,ZHAO Zhi-xun,YU Shao-xiong,YE Yi-you,ZHU Hai-xia,LI Jian,ZHANG Qiang. Function Prediction and Structure Analysis of N-terminal of E3L Protein in Sheeppoxvirus[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(6): 85-89 |
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| Authors: | LU Chang WU Guo-hua YAN Xin-min ZHAO Zhi-xun YU Shao-xiong YE Yi-you ZHU Hai-xia LI Jian ZHANG Qiang |
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| Affiliation: | 1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;2. Animal and Plant Inspection and Quarantine Technology Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen 518045, China |
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| Abstract: | E3L protein is a kind of nonstructural protein of poxvirus expressed in early time. It can inhibit immunity of host cells and be ralated with virus virulence and tropism. However, E3L protein in sheeppoxvirus (SPPV) is rarely understood. We have predicted N-terminal of E3L protein in SPPV by bioinformatics methods as well as using the N-terminal of E3L proteins which have been predicted. And then the structural domains, secondary structures, tertiary structures and amino acid compositions of the above proteins have also been predicted using these online programs, such as WebLab, SWISS-MODEL and ExPASY tools. The bioinformatical analysis results show that the protein and other Zα famliy proteins have the same basic structural characteristics and structural domains, implying that the N-terminal of E3L protein in SPPV might belong to Zα famliy. |
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| Keywords: | sheeppoxvirus E3L protein Z-DNA bioinformatics |
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