| 内蒙古地区绵羊肺炎支原体的分离鉴定及序列分析 |
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| 引用本文： | 张玲,郝永清,黄海碧,徐春光,程晨. 内蒙古地区绵羊肺炎支原体的分离鉴定及序列分析[J]. 中国畜牧兽医, 2013, 40(7): 158-161 |
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| 作者姓名： | 张玲 郝永清 黄海碧 徐春光 程晨 |
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| 作者单位： | 内蒙古农业大学兽医学院, 微生物学与免疫学实验室, 内蒙古呼和浩特 010018 |
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| 基金项目： | 内蒙古科技创新引导奖励基金(20101808)。 |
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| 摘 要： | 本试验无菌采取内蒙古地区某发病羊场的绵羊病变肺脏组织,接种于支原体液体培养基进行分离培养后获得1株支原体,根据分离株的培养特性、形态学观察及生化试验等,初步鉴定为绵羊肺炎支原体。然后提取分离株的基因组,用通用引物体外扩增出分离株16S rRNA序列,将该序列与GenBank中已知33种支原体序列进行比较,结果表明该序列与绵羊肺炎支原体标准株Y-98的16S rRNA序列的同源性为99%,鉴定该分离株为绵羊肺炎支原体。
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| 关 键 词： | 绵羊肺炎支原体 分离 鉴定 |
| 收稿时间： | 2012-11-15 |
Isolation,Identification and Sequence Analysis of Mycoplasma ovipneumoniae in Inner Mongolia |
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| ZHANG Ling,HAO Yong-qing,HUANG Hai-bi,XU Chun-guang,CHENG Chen. Isolation,Identification and Sequence Analysis of Mycoplasma ovipneumoniae in Inner Mongolia[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(7): 158-161 |
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| Authors: | ZHANG Ling HAO Yong-qing HUANG Hai-bi XU Chun-guang CHENG Chen |
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| Affiliation: | Laboratory of Microbiology and Immunology, College of Veterinary Science, Inner Mongolia Agricultural University, Hohhot 010018, China |
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| Abstract: | In this test we collected goat’s lesions of lung tissue in Inner Mongolia which was probably infected with pleuropneumonia,and inoculated it on the mycoplasma selection medium under sterile conditions to isolate Mycoplasma.Through the cultivated characters,morphology and culture characterization and biochemical reactions,the isolated strain was preliminarily identified as Mycoplasma ovipneumoniae(MO).Then we extracted the isolated strain’s genomic DNA and amplified its 16S rRNA by the commom primers,33 reference strains were loaded down from GenBank,the result of sequence analysis indicated that the isolated strain exhibited 99% homology with reference strain Y-98.The results indicated that the isolated strain was MO. |
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| Keywords: | Mycoplasma ovipneumoniae isolation identification |
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