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氯化胆碱诱导黄瓜抗白粉病机理研究
引用本文:陈夕军,沈世炜,陈银凤,张青,张家豪,张孝然,黄奔立.氯化胆碱诱导黄瓜抗白粉病机理研究[J].中国蔬菜,2013,1(18):81.
作者姓名:陈夕军  沈世炜  陈银凤  张青  张家豪  张孝然  黄奔立
作者单位:(;1. 扬州大学园艺与植物保护学院,江苏扬州 225009;;2. 扬州市邗江区植保植检站,江苏扬州225000;;3. 苏州市农业科学院,江苏苏州 215155)
基金项目:江苏省农业科技自主创新资金项目〔CX(12)3019〕,扬州大学博士启动基金项目
摘    要:氯化胆碱是一种新型广效的生长调节剂,以10 mmol·L-1 氯化胆碱处理黄瓜幼苗可明显提高
植株的株高、根长、鲜质量、干质量和叶绿素含量。诱导抗性机理研究表明:氯化胆碱处理后,黄瓜幼苗
体内β-1,3-葡聚糖酶、几丁质酶活性及富含羟脯氨酸糖蛋白、丙二醛含量均明显上升。以白粉病菌分
生孢子接种经氯化胆碱处理过的黄瓜叶片,结果显示分生孢子萌发率较低,芽管较短,且新产生的分生孢
子串数量较少。用氯化胆碱处理黄瓜植株,可延迟和减轻黄瓜白粉病的发生。

关 键 词:氯化胆碱  黄瓜  白粉病  诱导抗性  抗性机理  

Studies on Cucumber Resistance Mechanism to Powdery Mildew Induced by Choline Chloride
CHEN Xi-Jun,SHEN Shi-Wei,CHEN Yin-Feng,ZHANG Qing,ZHANG Jia-Hao,ZHANG Xiao-Ran,HUANG Ben-Li.Studies on Cucumber Resistance Mechanism to Powdery Mildew Induced by Choline Chloride[J].China Vegetables,2013,1(18):81.
Authors:CHEN Xi-Jun  SHEN Shi-Wei  CHEN Yin-Feng  ZHANG Qing  ZHANG Jia-Hao  ZHANG Xiao-Ran  HUANG Ben-Li
Institution:(;1.Horticulture and Plant Protection College, Yangzhou University, Yangzhou 225009, Jiangsu, China;;2.Hanjiang District Plant Protection Station, Yangzhou 225000, Jiangsu, China;;3.Suzhou City Aacademy of Agricultural Sciences,Suzhou 215155, Jiangsu, China)
Abstract:Choline chloride is a new growth regulator. When the cucumber seedlings were treated
with 10 mmol·L-1 choline chloride,their plant height,root length,dry weight,fresh weight,and
chlorophyll content were remarkably improved.Studies on induce resistance mechanism showed that the
content or activity of β-1,3-glucanase,chitinase,hydroxyproline rich glycoproteins( HRGP) and
malondialdehyde(MAD)of cucumber seedlings were significantly increased after being treated with
choline chloride.The conidiospores of Sphaerotheca fuliginea were inoculated on the cucumber leaves
treated with choline chloride.The results showed that the germination rate of conidiospores was decline.
The length of germ tubes was shorter and the number of new conidia strings was less than the contrast.
Choline chloride treatment on cucumber plants can delay and all eviate the occurrence of powdery mildew.
Keywords:Choline chloride  Cucumber  Powdery mildew  Induce resistance  Resistance mechanism  
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