Study on Proliferation Characteristics and Pathogenicity of Pseudorabies Virus Ra Strain in Different Cells

To select the most suitable cell line for research on the pathogenic mechanism and vaccine of pseudorabies virus (PRV) Ra strain, different kinds of cells (ST, PK-15, Vero, F81, DF1, MDCK and CEF) were chosen to be inoculated with PRV. Cytopathy effect (CPE) characteristics, time and titers of the cell cultures were compared in this study. According to the morphology observation of infected cells, we found that the PRV Ra strain could proliferate in these seven kinds of cells and cause significant CPE, and the CPE obviously appeared firstly with in 24 h on the PK-15 and ST cells. The TCID₅₀ of PRV in the seven kinds of cell cultures performed differently, and the TCID₅₀ of infected ST cell was the highest. Therefore, it suggested that ST cell could be used in the further study of PRV Ra strain in vitro.