鱼源维氏气单胞菌的耐药性研究

为检测7株鱼源维氏气单胞菌(Aeromonas veronii)的耐药基因和耐药表型的分布情况,试验采用PCR法检测分离株中氨基糖苷类耐药基因(aac(3)-Ⅱa、aac(6')-Ⅰb、ant(3")-Ⅰa和aph(3')-Ⅱa),磺胺类耐药基因(Sul1、Sul2和Sul3)和四环素类耐药基因(tetA、tetC和tetM),运用Kirby-Bauer纸片扩散法检测7株鱼源维氏气单胞菌分离株对22种常用抗生素的敏感性。结果表明,可检出耐药基因aac(3)-Ⅱa(71.4%)、aac(6')-Ⅰb(85.7%)、Sul2(85.7%)和tetA(28.5%);未检出ant(3")-Ⅰa、aph(3')-Ⅱa、Sul1、Sul3、tetC和tetM基因。7株维氏气单胞菌对磷霉素(100%)、多黏霉素B(100%)、痢特灵(85.7%)、奥复星(71.4%)较敏感;对氨苄西林(100%)、乙酰螺旋霉素(100%)、复方新诺明(85.7%)、磺胺异恶唑(85.7%)、四环素(85.7%)等耐药。这说明耐药基因和耐药表型之间存在一定的相关性。

Study on Drug Resistance in Aeromonas veronii Isolated from Different Fish

To detect the drug resistance genotype and phenotype produced by Aeromonas veronii isolated from different fish.PCR method was used to detect the aminoglycoside antibiotics resistance genes (aac (3)-Ⅱa, aac (6')-Ⅰb, ant (3")-Ⅰa, aph (3')-Ⅱa), sulfonamides resistance genes (Sul1, Sul2, Sul3) and tetracyclines resistance genes (tetA, tetC, tetM) in isolates. The drug susceptibility to seven Aeromonas veronii strains toward 22 kinds of antibiotics were performed with Kirby-Bauer disc diffusion method. The results showed that the detected resistance genes were aac (3)-Ⅱa (71.4%), aac (6')-Ⅰb (85.7%), Sul2 (85.7%) and tetA (28.5%),while the other resistant genes of ant (3")-Ⅰa, aph (3')-Ⅱa, Sul1, Sul3, tetC and tetM were not checked out. The seven Aeromonas veronii isolates were sensitive to Fosfomycin (100%), Polymyxin B (100%), Furazolidone (85.7%) and Ofloxacin (71.4%). They were resistant to Ampicillin (100%), Acetylspiramycin (100%), Trimethoprim/Sulfamethoxazole (85.7%), Sulfisoxazole (85.7%) and Tetracycline (85.7%). Drug resistance genotype had some correlation with the drug resistance phenotype.