| 槟榔江水牛群体遗传结构的RAPD分析 |
| |
| 引用本文： | 李大林,霍金龙,霍海龙,苗永旺,屈在久,尹玉安. 槟榔江水牛群体遗传结构的RAPD分析[J]. 云南农业大学学报(自然科学版), 2007, 22(3): 340-344 |
| |
| 作者姓名： | 李大林 霍金龙 霍海龙 苗永旺 屈在久 尹玉安 |
| |
| 作者单位： | 云南巴福乐水牛技术研究所，云南农业大学动物科学技术学院，云南农业大学动物科学技术学院，云南农业大学动物科学技术学院，云南省腾冲县畜牧局，云南省腾冲县畜牧局 云南昆明650021，云南昆明650201，云南昆明650201，云南昆明650201，云南大学，生物资源保护与利用国家重点实验室，云南昆明650091，云南腾冲679100，云南腾冲679100 |
| |
| 基金项目： | 国家自然科学基金项目(30660024)，云南省科技厅应用基础研究计划面上项目(2006C0034M)。 |
| |
| 摘 要： | 为探讨RAPD标记在水牛遗传多样性方面检测的应用价值，以及了解槟榔江水牛的群体遗传结构状况，研究采用随机扩增多态性DNA标记技术对槟榔江水牛20个个体进行了遗传变异分析，从70个随机引物中筛选出15个多态性丰富的引物对其所有个体的总基因组DNA进行了PCR扩增，结果15条引物共产生105种扩增片段，多态片段95条，平均每条引物的扩增带数为7，各引物多态性片段范围在2～12之间，多态频率在40%～100%之间，多态座位平均为90.48%。表明槟榔江水牛的遗传多样性较丰富。
|
| 关 键 词： | 槟榔江水牛 随机扩增多态DNA 遗传多样性 |
| 文章编号： | 1004-390X（2007）03-0340-05 |
| 收稿时间： | 2006-08-30 |
| 修稿时间： | 2006-08-30 |
Analysis of Population Genetic Structure of Binlangjang Water Buffalo using RAPD Markers |
| |
| LI Da-lin,HUO Jin-long,HUO Hai-long,MIAO Yong-wang,QU Zai-jiu,YIN Yu-an. Analysis of Population Genetic Structure of Binlangjang Water Buffalo using RAPD Markers[J]. Journal of Yunnan Agricultural University, 2007, 22(3): 340-344 |
| |
| Authors: | LI Da-lin HUO Jin-long HUO Hai-long MIAO Yong-wang QU Zai-jiu YIN Yu-an |
| |
| Affiliation: | 1. Yunnan Institute of Buffalo Science and Technology, Kunming 650021, China; 2. Faculty of Animal Sciences, Y A U, Kunming 650201, China; 3. Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, China; 4. Bureau of Stock Raising and Veterinary Medicine of Tengchong County, Tengchong 679100, China |
| |
| Abstract: | In order to estimate the value of the RAPD marker on the study of water buffalo population genetic diversity,as well as the genetic construction on Binglangjiang water buffalo population,the random amplified polymorphic DNA(RAPD) marker was used to analyze genetic variation of 20 Binglangjiang water buffaloes in this paper.Individual genome DNA was amplified with 15 polymorphism random primers which were screened from 70 random primers.105 bands were obtained,of which 95 bands were polymorphic,each primer had 7 bands on average and the fragment number is in between 2~12,frequencies of polymorphic bands is in between 40%~100%,the mean value of polymorphic loci was 90.48%. The results indicated that the genetic diversity of Binglangjiang water buffalo is rich. |
| |
| Keywords: | Binglangjiang waterbuffalo random amplified polymorphic DNA(RAPD) genetic diversity |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《云南农业大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《云南农业大学学报(自然科学版)》下载全文 |
