Particle Size Distribution in Two Lipid Emulsions Used for the Enrichment of Artemia nauplii as a Function of Their Preparation Method and Storage Time |
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| Authors: | Kyungmin Han Inge Geurden Paul Van der Meeren Sungchul C. Bai Patrick Sorgeloos |
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| Affiliation: | Laboratory of A quaculture and Artemia Reference Center, Ghent University, Rozier 44, 9000 Gent, Belgium;Fish Nutrition Laboratory INRA-IFREMER, B.P. 3. 64310 St. Pée-sur-Nivelle, France;Department of Applied Analytical and Physical Chemistry, Particle and Interface Technology Group, Ghent University, Coupure links 653, B-9000 Gent, Belgium;Department of Aquaculturel Feeds &Foods Nutrition Research Center, Pukyong National University, Busan 608–737 Korea;Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Rozier 44, 9000 Gent, Belgium |
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| Abstract: | The present study examined the distribution of particle sizes in two experimental standardized lipid emulsions (distributed by ICES, International Council for the Exploration of the Sea) as a function of the preparation method (hand shaking or ultrasonic blending) and as a function of storage time. A 24-h enrichment trial compared the incorporated HUFA levels in the nauplii of Artemia franciscana .
The emulsion droplets in the 50% HUFA emulsion (ICES 50, ethyl ester based) were much smaller than in the 30% HUFA emulsion (ICES 30, triacylglycerol-based) in which 90% of the droplets had a diameter below 12.3 μm as compared to 1.3 pn in ICES 50. The blending method highly affected particle sizes. High-shear blending instead of the classical hand shaking of the emulsion of both ICES 30 and 50 reduced the mean particle size from 5.06 to 1.07 μm and from 0.38 to 0.11 μm, respectively. The particle size distributions remained stable during the 1-wk storage, suggesting the absence of agglomeration or bacterial development. The fatty acid composition of 24-h enriched Artemia reflected differences in the HUFA profile of the emulsions, but was independent of observed differences in the size of the particles. |
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