高效液相色谱法测定乳品与饲料中阿维菌素类药物残留

建立乳品与饲料中阿维菌素类药物(埃普利诺菌素、阿维菌素、多拉菌素和伊维菌素)残留液相色谱的测定方法。乙腈为提取剂,tC18固相萃取小柱净化,三氟乙酸酐和N-甲基咪唑柱前衍生,C18柱分离,甲醇-乙腈的混合溶剂与水梯度洗脱,液相色谱仪荧光检测器检测,外标法定量。每1 000 g乳粉(或每1 000 mL液态乳)中阿维菌素添加量为1.5 μg时,回收率大于95％。本方法液态乳、酸乳、乳粉的定量限为2μg/kg,饲料的定量限为10 μg/kg。该方法具有结果准确、操作简便、重复性好等优点,可用于乳品和饲料中阿维菌素类药物残留的检测。

High Performance Liquid Chromatography Determination of Abamectin Residues in Dairy Products and Feed

A method for the determination of abamectin residues in dairy products and feed by using high performance liquid chromatography (HPLC) with fluorescence detection after precolumn derivation with trifluoroacetic anhydride and N-methyl imidazole was developed.Abamectin residues were extracted with acetonitrile,cleaned up on a tC18 solid phase extraction cartridge.After precolumn derivatization,the separation was performed on C18 column by gradient elution with methano-acetonitrile-water as mobile phase.The analyte were detected with a fluorescence detector and quantified by an external standard method.The recovery of abamectins spiked into milk powder at 1.5 μg/1 000 g (or 1 000 mL for milk) was higher than 90％.The limit of quantitation was 2 μg/kg for dairy products and 10.0 μg/kg for feed.This method proved to be accurate,simple reproducible,and suitable for the analysis of abamectin residues in dairy products and feed.