马铃薯StDRO1基因的多态性鉴定及其与根系性状的关联分析

为鉴定与马铃薯根系性状相关联的StDRO1基因单核苷酸多态性(single nucleotide polymorphism, SNP)位点,本研究对110份同源四倍体马铃薯材料StDRO1基因的编码区进行了克隆和测序,筛选其SNP,并对StDRO1的SNP位点与马铃薯总根表面积、总根体积和平均根系直径等主要根系性状参数进行了关联分析。结果显示,StDRO1第2外显子上检测到1个SNP位点,命名为G64C;第3外显子上检测到10个SNP位点,分别命名为G152A、A214G、A297G、C314T、A337T、T353C、T560A、C577A、C620A和C625A;在第4外显子上检测到1个SNP位点,命名为T793A。关联分析结果表明,StDRO1基因G152A位点在总根体积表现为GA类基因型>GG类基因型(P<0.05);C314T位点在平均根系直径表现为CC类基因型>CT类基因型(P<0.05); A337T位点在总根表面积、鲜重和干重均表现为AT类基因型>AA类基因型(P<0.05),而在平均根系直径则表现为AA类基因型>AT类基因型(...

Identification of StDRO1 gene polymorphism and association analysis with root traits in potato

In order to identify the single nucleotide polymorphism(SNP)of StDRO1 gene associated with potato root traits,StDRO1 gene coding region of the 110 tetraploid potato genotypes was cloned and sequenced.The association analysis between the StDRO1 SNPs and the major root traits of potato such as surface area,volume,and average diameter were carried out.The results showed that a SNP(G64C)was detected in the second exon of the StDRO1,10 SNPs(G152A,A214G,A297G,C314T,A337T,T353C,T560A,C577A,C620A,and C625A)were identified in the third exon,and a SNP(T793A)was detected in the fourth exon.The association analysis indicated that in the total root volume,G152A locus of StDRO1 gene showed that GA geno-type was superior to GG genotype(P<0.05).In the average root diameter,C314 locus showed that CC genotype were superior to CT genotype(P<0.05).In the root surface area,fresh weight,and dry weight,A337T locus showed that AT genotype were better than AA genotype(P<0.05),while the locus in the average root diameter showed that AT genotype was significantly lower than AA genotype(P<0.05).The root surface area,root volume,and fresh weight were showed as TC genotype>TT genotype(P<0.05)in T353C site;the root volume was showed as CA genotype>CC genotype(P<0.05)in C620A site;the root surface area,root volume,and fresh weight were showed as AT genotype>TT genotype(P<0.05)in T793A site.In summary,the above six SNPs of StDRO1 genes had a significant impact on potato root traits,and the A337T,T353C,and T793A sites were particularly important.These results provide a basic theoretical reference for subsequent potato root architecture research and genetic im-provement,but whether it could be used as a genetic marker for potato root traits need to be further verified by expanding the population sample.