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肉牛口蹄疫免疫抗体、3ABC抗体、RT-PCR核酸检测结果分析
引用本文:朵玉明,何彦春.肉牛口蹄疫免疫抗体、3ABC抗体、RT-PCR核酸检测结果分析[J].中国牛业科学,2019,45(6):29-30.
作者姓名:朵玉明  何彦春
作者单位:甘肃省肃南县畜牧兽医技术服务中心,甘肃肃南734000;张掖市动物疫病预防控制中心,甘肃张掖734000
摘    要:目的]为了掌握肉牛口蹄疫免疫抗体检测结果、口蹄疫非结构蛋白3ABC抗体检测结果、口蹄疫O-P液RT-PCR核酸检测结果三者的相关性,方法]张掖市动物疫病预防控制中心对甘州区28头肉牛开展相关检测。结果]检测结果表明:O型口蹄疫免疫抗体合格率28份,合格率100%,3ABC抗体阳性27份,阳性率96.4%;对3ABC抗体阳性的27头牛分2次进行实时荧光RT-PCR核酸检测,被检的27头牛均为阴性。

关 键 词:肉牛  口蹄疫  免疫抗体  3ABC抗体  RT-PCR核酸检测

Analysis of FMD Antibody,3ABC Antibody and RT-PCR Nucleic Acid Detection Results in Beef Cattle
DUO Yu-ming and HE Yan-chun.Analysis of FMD Antibody,3ABC Antibody and RT-PCR Nucleic Acid Detection Results in Beef Cattle[J].China Cattle Science,2019,45(6):29-30.
Authors:DUO Yu-ming and HE Yan-chun
Institution:The Technical Service Center of Animal Husbandry and Veterinary,Sunan County ,Sunan,Gansu 734000 and Zhangye Animal Disease Prevention and Control Center,Zhangye,Gansu 734000
Abstract:Objective] In order to master the correlation among the detection results of foot and mouth disease (FMD) immune antibody, FMD nonstructural protein 3ABC antibody and FMD O-P solution RT-PCR nucleic acid, Method] Zhangye animal disease prevention and control center carried out the relevant detection on 28 beef cattle in Ganzhou district. Result] The results showed that the qualified rate of O-FMD antibody was 28, the qualified rate was 100%, the positive rate of 3ABC antibody was 27, the positive rate was 96.4%; 27 cattle with 3ABC antibody positive were detected by real-time RT-PCR twice, and all 27 cattle were negative.
Keywords:beef cattle  foot and mouth disease  immune antibody  3ABC antibody  RT-PCR nucleic acid detection
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