集约化养猪场嗜流产衣原体病流行状况的初步调查

本试验对北京郊区5个规模化猪场断奶仔猪、育成猪、育肥猪和怀孕母猪采集血清共507份,同时采集密切接触饲养人员、仔猪、育肥猪的喉头拭子、母猪阴道拭子及公猪的精液共183份。分别使用间接血凝诊断试剂盒、法国ELISA试剂盒检测抗体;利用直接荧光染色法检测抗原,包括166份猪喉头拭子、阴道拭子和精液以及17份饲养人员喉头拭子。结果发现间接血凝诊断试剂盒、ELISA试剂盒抗体阳性率分别为4.14%和2.17%;抗原平均阳性率为14.8%,其中精液阳性率达到37.5%,母猪阴道拭子阳性率为27.5%,饲养人员阳性率为23.5%。本试验证实北京郊区猪嗜流产衣原体在种公猪、母猪群和饲养员呈高流行趋势。同时研究结果显示,5个被调查的规模化猪场嗜流产衣原体抗体阳性率显著低于有报道的其他省市,这与间接血凝诊断试剂盒检测结果高于ELISA试剂有关。针对发现的问题,必须采取有效措施控制种公猪精液污染衣原体现象,阻断向母猪和饲养人员传播,以降低人兽共患病发生的风险。

Preliminary Investigation of Chlamydophila abortus in Intensive Pig Farms

In order to evaluate prevalence of swine Chlamydophila abortus in Beijing intensive pig farms,507 blood samples were detected for antibodies using indirectly hemorrhage assay(IHA) and ELISA while 183 clinical samples were determined for positive antigens by immunofluorescence test kits(IF),including 166 samples from pig swabs and pig sperms and 17 throat swabs from pig farmers.The positive antibody against Chlamydophila abortus was 4.14%(IHA) and 2.17%(ELISA),respectively while the average positive antigen was average 14.8%(IF).Moreover,the higher antigen positivity was found in the boar sperm samples,sow virginal swabs and in throat swabs from pig farmers,which accounted for 37.5%,27.5% and 23.5%,respectively in the survey.On contrary to the previous reports,the positive antibody in Beijing intensive pig farms was found to be lower prevalence than the other province.Our investigation firstly explored that Chlamydophila abortus was prevalence in intensive pig farms as well as in the closely human beings.The urgent measure should be taken into account for cutting transmission from boar sperms to sows and human beings.