| 利用MALDI-TOF质谱比较水牛卵泡液多肽变化的研究 |
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| 引用本文： | 付强,刘振方,杨丽,张明,卢克焕.利用MALDI-TOF质谱比较水牛卵泡液多肽变化的研究[J].中国畜牧兽医,2012,39(1):132-136. |
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| 作者姓名： | 付强 刘振方 杨丽 张明 卢克焕 |
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| 作者单位： | 广西大学动物繁殖研究所,亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004 |
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| 基金项目： | 国家自然科学基金，广西科技厅基础条件平台建设，高等学校博士点科研基金 |
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| 摘 要： | 为检测卵泡内小肽的表达,采用基质辅助激光解离串联飞行时间质谱(MALDI-TOF/TOF MS)建立了成熟卵泡液的多肽表达模型,比较小卵泡(直径<4 mm)、中等卵泡(直径4~8 mm)和成熟卵泡(直径>8 mm)的多肽表达变化。结果表明,3种形态的卵泡液存在12个显著差异多肽,其中在成熟卵泡内上调6个,下调有5个;在中等卵泡内上调4个,下调5个。其中分子质量约为1746 u的多肽在成熟卵泡中特异性表达,可作为卵泡成熟的潜在生物标志物之一。
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| 关 键 词： | 水牛 卵泡液 MALDI-TOF 多肽 特异性表达 |
| 收稿时间： | 2011-06-01 |
Comparison of Peptides Expression in Buffalo Follicular Fluid with MALDI-TOF Mass Spectrometry |
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| FU Qiang
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LIU Zhen-fang
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YANG Li
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ZHANG Ming
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LU Ke-huan.Comparison of Peptides Expression in Buffalo Follicular Fluid with MALDI-TOF Mass Spectrometry[J].China Animal Husbandry & Veterinary Medicine,2012,39(1):132-136. |
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| Authors: | FU Qiang LIU Zhen-fang YANG Li ZHANG Ming LU Ke-huan |
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| Institution: | State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Animal Reproduction Institute, Guangxi University, Nanning 530004, China |
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| Abstract: | In order to detect the peptides expression profile in follicles,the matrix-assisted laser desorption ionization mass spectrometry was used to establish a model of peptide expression profile and compare expression changes of peptides from small follicles((φ<4 mm),medium follicules(4 to 8 mm) and mature follicles(φ>8 mm).The results indicated that there were 12 peptides to express significantly different among three shapes of follicles.6 peptides increased and 5 peptides decreased in mature follicles.The 1746 u of peptide expressed specifically in mature follicles,which might be used as the one of the underlying biomarkers for follicles maturation. |
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| Keywords: | buffalo follicular fluid MALDI-TOF peptide specific expression |
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