石榴皮提取物对离体培养兔球虫卵囊的杀灭作用

本试验旨在研究石榴皮提取物对离体培养兔球虫卵囊的杀灭作用。采集已感染球虫的18只家兔(体重2.0~2.5 kg)的新鲜粪便(24 h内,35 g/只),用饱和盐水漂浮法分离、提取球虫卵囊。球虫卵囊分别在蒸馏水、石榴皮水提物、石榴皮黄酮、石榴皮多酚、盐酸氯苯胍中培养24、48、72 h,光镜下观察不同浓度的各种药物对兔球虫卵囊数量的影响。结果显示,与蒸馏水组比较,5、10 mg/mL剂量组培养72 h,4种药物均使兔球虫卵囊数量明显减少。球虫卵囊的减少率石榴皮水提物组大于石榴皮黄酮组,石榴皮黄酮组大于石榴皮多酚组和盐酸氯苯胍组;起效时间约为48~72 h。1 mg/mL剂量组4种药物虽可使兔球虫卵囊数量减少,但无显著差异(P＞0.05)。结果提示,石榴皮提取物对体外培养的兔球虫卵囊有较强的杀灭作用,其中石榴皮水提物对兔球虫卵囊杀灭作用最强,石榴皮黄酮和石榴皮多酚次之,其杀灭作用呈一定的正相关量效关系。

The Extermination Effect of Pomegranate Peel Extracts on Rabbit Coccidian Oocysts Cultured in Vitro

The exterminatory actions of pomegranate peel extract to rabbit coccidia oocysts cultured in vitro were studied in this paper.We collected the excrement(24 h,35 g each rabbit) from eighteen rabbits infected with coccidian,weighing 2.0 to 2.5 kg,separated and extracted the coccidia oocysts with saturation salt water floating method.The coccidia oocysts were cultivated in distilled water,aqueous extract of pomegranate peels,pomegranate flavonoids,pomegranate polyphenols and Robenidine HCl for 24,48 and 78 h,respectively.The number of rabbit coccidia oocysts was observed with the optical microscope.The effects of different concentrations of drugs and different cultivated time on the amount of rabbit coccidia oocysts were estimated.The results showed that comparing with distilled water group,the amount of rabbit coccidia oocysts separately cultivated in 72 h and 5,10 mg of the four drugs groups reduced,and the reducing number ranged in descending order was aqueous extract of pomegranate peels group,pomegranate flavonoids group,pomegranate polyphenols group and Robebidine HCl group.The onset time of the extracts of pomegranate peel were 48 and 72 h.The amount of rabbit coccidia oocysts cultivated in 24,48,72 h and 1 mg of the four drugs groups were not reduced significantly(P>0.05).There were many notes which indicated that the extracts of pomegranate peel were the stronger exterminatory action to the rabbit coccidia oocysts cultured in vitro.The exterminatory action of aqueous extract to the rabbit coccidia oocysts was the strongest,followed by pomegranate polyphenols and pomegranate flavonoids in the 3 extractions from pomegranate peel.The exterminatory action had a certain positive correlation with the dose.