| 桑树低温诱导基因Wap25的序列分析及表达产物的亚细胞定位 |
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| 引用本文： | 陆小平,刘嘉琦,李叶峰,楼程富.桑树低温诱导基因Wap25的序列分析及表达产物的亚细胞定位[J].蚕业科学,2010,36(2):209-213. |
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| 作者姓名： | 陆小平 刘嘉琦 李叶峰 楼程富 |
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| 作者单位： | 苏州大学金螳螂建筑与城市环境学院,江苏苏州,215123;浙江大学动物科学学院,杭州,310029 |
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| 基金项目： | 国家自然科学基金项目，公益性行业(农业)科研专项项目 |
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| 摘 要： | Wap25是从桑树幼茎克隆的低温诱导蛋白基因,为胚胎后期富集蛋白(LEA)基因的成员之一,与其它物种低温诱导基因的同源性很低。生物信息学分析表明,桑树低温诱导基因Wap25编码蛋白由226个氨基酸组成,分子质量25.3kD,等电点5.52;蛋白N端1-30位氨基酸表现出强烈的疏水性,其后是亲水性高的区域,平均亲水值达-1.020,而蛋白1-29位氨基酸为典型的真核生物蛋白质的信号肽序列,表明WAP25蛋白属于分泌型蛋白。用SubLocv1.0软件预测WAP25的亚细胞定位,将该蛋白定位于细胞质中。Tmpred软件预测WAP25不存在跨膜结构。构建Wap25与绿色荧光蛋白(GFP)的重组质粒,用基因枪法将其转入洋葱表皮细胞,在激光扫描共聚焦显微镜下发现GFP分散于洋葱表皮的细胞质中,而在细胞核未发现GFP的表达,此结果与WAP25的亚细胞定位预测结果相符。
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| 关 键 词： | 桑树 蒙古桑 低温诱导基因Wap25 序列分析 亚细胞定位 绿色荧光蛋白 |
Sequence Analysis of Cold-induced Gene Wap25 in Morus mongolica and Subcellular Localization of its Expression Product |
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| LU Xiao-Ping,LIU Jia-Qi,LI Ye-Feng,LOU Cheng-Fu.Sequence Analysis of Cold-induced Gene Wap25 in Morus mongolica and Subcellular Localization of its Expression Product[J].Acta Sericologica Sinica,2010,36(2):209-213. |
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| Authors: | LU Xiao-Ping LIU Jia-Qi LI Ye-Feng LOU Cheng-Fu |
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| Institution: | LU Xiao-Ping1 LIU Jia-Qi1 LI Ye-Feng1 LOU Cheng-Fu2(1Golden Mantis School of Architecture , Urban Environment,Soochow University,Suzhou Jiangsu 215123,China,2College of Animal Sciences,Zhejiang University,Hangzhou 310029,China) |
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| Abstract: | Wap25 gene,which belongs to the late embryogenesis abundant(LEA)family,was cloned from young stem of mulberry.The alignment analysis showed that this gene had very low identities with cold-induced genes in other species.Bioinformatics analyses indicated that Wap25 encodes a 226 amino acid protein with a theoretical molecular mass of 25.3 kD and a pI of 5.52.Its N-terminal amino acids 1 to 30 have strong hydrophobicity.The following amino acids are highly hydrophilic,with an average hrdrophilicity score of-1... |
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| Keywords: | Morus L Morus mongolica Cold-induced gene Wap25 Sequence analysis Subcellular localization Green fluorescent protein |
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