| 提高大麦游离小孢子培养反应的研究 |
| |
| 引用本文： | 陆瑞菊,孙月芳,王亦菲,周润梅,黄剑华. 提高大麦游离小孢子培养反应的研究[J]. 大麦与谷类科学, 2004, 0(2): 14-16 |
| |
| 作者姓名： | 陆瑞菊 孙月芳 王亦菲 周润梅 黄剑华 |
| |
| 作者单位： | 上海市农科院生物技术研究中心,上海,201106,上海市农业遗传育种重点实验室;上海市农科院生物技术研究中心,上海,201106,上海市农业遗传育种重点实验室;上海市农科院生物技术研究中心,上海,201106,上海市农业遗传育种重点实验室;上海市农科院生物技术研究中心,上海,201106,上海市农业遗传育种重点实验室;上海市农科院生物技术研究中心,上海,201106,上海市农业遗传育种重点实验室 |
| |
| 摘 要： | 提取液中添加 10mg/L秋水仙碱可提高大麦小孢子游离后的存活率 ;以含 10mg/L秋水仙碱的预处理液预处理小孢子 4 8h可以降低培养 72h后小孢子的死亡率 ;以小花共培养可以提高正常型多细胞结构形成率 ,因而明显提高胚状体的形成。
|
| 关 键 词： | 大麦 小孢子培养 秋水仙碱 小花共培养 |
| 修稿时间： | 2004-03-28 |
Studies on Improving Response of Microspore Culture in Barley |
| |
| Lu Ruiju Sun Yuefang Wang Yifei Zhou Runmei Huang Jianhua. Studies on Improving Response of Microspore Culture in Barley[J]. Barley and Cereal Sciences, 2004, 0(2): 14-16 |
| |
| Authors: | Lu Ruiju Sun Yuefang Wang Yifei Zhou Runmei Huang Jianhua |
| |
| Abstract: | The frequency of survival isolated microspores could be increased by adding colchicine 10 mg/L to isolation solution. The frequency of dead microspores after 72h culturing could be becreased by pretreatment of freshly isolated microspores with isolation solution supplemented with colchicine 10 mg/L for 2d. The productions of multicellular structure and embryo like structure were improved by co culturing with florets of barley. |
| |
| Keywords: | Barley Micropsores culture Colchicine Co Culture with florets |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
