陆地棉耐盐相关基因GhERF79的克隆与分析

利用RACE-PCR技术克隆陆地棉ERF基因的全长cDNA,命名为GhERF79,该cDNA长度为980 bp,含有一个完整的ORF(Open reading frame)为756 bp,编码251个氨基酸的多肽;亚细胞定位显示该基因表达产物定位于细胞核中,生物信息学分析表明GhERF79属于ERF转录因子家族内的A-1亚族。Real-time PCR分析结果表明,GhERF79的表达受盐胁迫和植物激素诱导,该基因在耐盐材料中9806中的诱导表达水平显著高于盐敏感材料中棉所12;外源激素ABA(Abscisic acid)、ET(Ethylene)、MeJA(Methyl jasmonate)可以显著诱导GhERF79的表达,推测其可能在棉花响应盐胁迫途径中发挥重要功能。

Cloning and Analysis of Salt-tolerance Ralated Gene GhERF79 from Gossypium hirsutum L.

 In this study, the full-length cDNA sequence of GhERF79 was isolated from Gossypium hirsutum by RACE-PCR, its length is 980 bp, and it has an open reading frame (ORF) of 756 bp encoding a protein of 251 amino acids which was located in nucleus by subcellular localization experiment; Bioinformation analysis revealed that GhERF79 belonged to A-1 subgroup of ERF subfamily; Expression pattern analysis revealed that the expression of GhERF79 was significantly induced by salt stress and plant hormones ABA, ET, and MeJA, furthermore, the induced expression level in salt-tolerance germplasm Zhong 9806 (G. hirsutum) was considerably higher than that in salt-sensitive cultivar CCRI 12 (G. hirsutum). The evidence above suggested that GhERF79 might play an important role in regulating cotton salt stress responses.