Erwinia species in the lenticels of certified seed potatoes

Twelve-tuber samples were collected from 39 certified seed stocks grown in 4 states and a Canadian province. One-half of each sample was immersed in water and the other half exposed to a CO₂-N atmosphere at 25C to induce soft rot development at the lenticels. Pectolytic bacteria were isolated from 24 seed stocks which represented each producing area. The pathogenicity and biochemical reactions of 20 isolates were those ofErwinia carotovora var.atroseptica, except for one isolate which grew at 37C. Four isolates did not cause black leg but only three reacted biochemically likeE. carotovora var.carotovora. The results confirm reports that theErwinia spp. are tuber borne and that anaerobic conditions stimulate infection of naturally inoculated lenticels.