| 板栗种仁中总酚酸的提取纯化工艺研究 |
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| 引用本文： | 马宏峰,孙志,王凤春,杨军,赵亚男,吴立军,高慧媛. 板栗种仁中总酚酸的提取纯化工艺研究[J]. 安徽农业科学, 2012, 0(36): 17721-17722 |
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| 作者姓名： | 马宏峰 孙志 王凤春 杨军 赵亚男 吴立军 高慧媛 |
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| 作者单位： | 1. 河北省迁西县板栗产业研究发展中心,河北省板栗工程中心,河北迁西 064300
2. 沈阳药科大学中药学院,辽宁沈阳,110016 |
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| 基金项目： | 国家林业公益性行业科研专项项目(201204401) |
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| 摘 要： | [目的]建立富集板栗种仁中高纯度总酚酸的方法.[方法]以没食子酸为对照品,采用分光光度法测定板栗种仁中总酚酸含量；以总酚酸含量为指标,对提取方法进行系统研究；以总酚酸的得率和精制度为考察指标,研究大孔树脂吸附纯化提取物的工艺条件及参数.[结果]用体积分数为75％的乙醇回流提取的总酚酸获得较高的提取率,通过HPD-826型大孔树脂纯化后,总酚酸得率的质量分数达到83.6％以上,体积分数为70％的乙醇洗脱液总固物中总酚酸的质量分数达到58.1％以上.[结论]采用此种提取及精制方法,可较好地提取纯化板栗种仁中的总酚酸.
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| 关 键 词： | 板栗种仁 总酚酸 提取 纯化工艺 分光光度法 大孔树脂 |
Investigation on Extraction and Purification of Total Phenolic Acid in Castanea mollissima Blume |
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| Affiliation: | MA Hong-feng et al(Center of Qianxi County Chestnut Industry Development,Hebei Chestnut Engineering Center,Qianxi,Hebei 064300) |
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| Abstract: | [Objective] To establish an enrichment method of high phenolic acid in Castanea mollissima Blume.[Method] Phenolic acid was monitored by UV method to investigate the extraction and enrichment in Castanea mollissima Blume.[Result] The optimum extraction composition was 75% ethanol.After the purification of macroporous resin,the elution ratio of phenolic acid was up to 83.6%,the amount of phenolic acid in total solidified extract was more than 58.1% when 70% ethanol was used as elution solvent.[Conclusion] This extraction and purification method can be applied to extract high purification phenolic acid from Castanea mollissima Blume. |
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| Keywords: | Castanea mollissima Blume Total phenolic acid Extraction Purification technology UV Macroporous resin |
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