| FKBP38条件性基因敲除胚胎干细胞株的建立 |
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| 引用本文： | 马贯中,吕萌. FKBP38条件性基因敲除胚胎干细胞株的建立[J]. 安徽农业科学, 2012, 0(36): 17611-17613 |
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| 作者姓名： | 马贯中 吕萌 |
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| 作者单位： | 南京医科大学江苏省医药实验动物基地,江苏南京,210019;南京医科大学江苏省医药实验动物基地,江苏南京,210019 |
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| 摘 要： | [目的]通过电转的方法,建立FKBP38细胞株.[方法]对自制的MEF细胞进行MMC处理,从而得到Feeder细胞,并在Feeder细胞上培养ES细胞.[结果]通过电转的方法,将FKBP38载体转入ES细胞中,经G418,Ganc筛选克隆和PCR检测,得到FKBP38阳性克隆.[结论] FKBP38 CKO胚胎干细胞株构建完成.
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| 关 键 词： | 干细胞 电转 FKBP38 |
Establish CKO FKBP38 ES cell line |
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| Affiliation: | MA Guan-zhong et al(Medical Experimental Animal Base of Nanjing Medical University,Nanjing,Jiangsu 210019) |
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| Abstract: | [Objective] To construct FKBP38 cell line through electroporation.[Method] We treated the MEF with MMC,and culture ES cells on the feeder dish to get feeder cell.Through electroporation and screening by G418 and Ganc,we got some clones and detected the clones by PCR.[Result] We got the positive FKBP38 cell line.[Conclusion] The CKO embryonic stem cell lines of FKBP38 were successfully built. |
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| Keywords: | ES cell Electroporation FKBP38 |
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