| PVP对烟草基因组DNA提取的影响 |
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| 引用本文： | 邓力超,邱道寿,屠乃美,谭铭喜,张振臣,陈俊标,李淑玲,王泽清.PVP对烟草基因组DNA提取的影响[J].广东农业科学,2009(5):37-39,57. |
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| 作者姓名： | 邓力超 邱道寿 屠乃美 谭铭喜 张振臣 陈俊标 李淑玲 王泽清 |
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| 作者单位： | 邓力超(广东省农科院作物研究所/农业部华南农作物种质资源利用重点开放实验室,广东广州510640;湖南农业大学农学院,湖南长沙,410128);邱道寿,谭铭喜,张振臣,陈俊标,李淑玲,王泽清(广东省农科院作物研究所/农业部华南农作物种质资源利用重点开放实验室,广东广州,510640);屠乃关(湖南农业大学农学院,湖南长沙,410128) |
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| 基金项目： | 广东省农科院科技支撑项目，广东省烟草专卖局科技计划项目 |
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| 摘 要： | 为克服烟叶组织中蛋白质、糖类、色素、烟碱及酚类等物质对烟草基因组DNA提取的干扰,防止DNA产生褐变,获得质量高且适于SRAP-PCR反应需要的DNA,采用改良CTAB法探讨了在提取液中添加不同浓度PVP对烟草基因组DNA提取质量的影响.结果表明:在1%、2%、4%PVP浓度下提取烟草DNA样品的OD260/OD280值均在1.7～1.9之间,OD260/OD280值随着提取液中PVP浓度的提高而增大,DNA褐化程度明显降低,获得的DNA能满足SRAP分析要求.
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| 关 键 词： | 烟草 DNA提取 |
Effect of PVP on the Extraction of Tobacco Genomic DNA |
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| DENG Li-chao,QIU Dao-shou,TU Nai-mei,TAN Ming-xi,ZHANG Zhen-chen,CHEN Jun-biao,LI Shu-ling,WANG Ze-qing.Effect of PVP on the Extraction of Tobacco Genomic DNA[J].Guangdong Agricultural Sciences,2009(5):37-39,57. |
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| Authors: | DENG Li-chao QIU Dao-shou TU Nai-mei TAN Ming-xi ZHANG Zhen-chen CHEN Jun-biao LI Shu-ling WANG Ze-qing |
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| Institution: | DENG Li-chao1,2,QIU Dao-shou1,TU Nai-mei2,TAN Ming-xi1,ZHANG Zhen-chen1,CHEN Jun-biao1,LI Shu-ling1,WANG Ze-qing1(1.Crop Research Institute,Guangdong Academy of Agricultural Sciences/ Key Laboratory of South China Crop Germplasm Enhancement,Ministry of Agriculture,Guangzhou 510640,China,2.College of Agronomy,Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | In order to eliminate the interference of tobacco leave tissue protein,carbohydrate,pigment,nicotine and phenol to the extraction of genomic DNA,prevent DNA from browning,and obtain high quality DNA template for SRAP-PCR analysis,a modified CTAB method was used in this paper to investigate the effect of Polyvinylpyrrolidone(PVP) concentrations added in extracting buffer solution on the extraction of tobacco genomic DNA.The results indicated that the OD260/OD280 ratio of extracted DNA was ranged from 1.70 to... |
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| Keywords: | PVP SRAP |
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