丝状霉形体丝状亚种SC型脂蛋白LppQ N末端基因克隆与序列分析

脂蛋白LPPQ是丝状霉形体丝状亚种SC型(MmmSC)非洲株、欧洲株和疫苗株所特有的。LPPQ N末端域具有良好的免疫原性，在牛体内可诱导产生强大、特异、早期、持续的免疫反应。本研究根据已发表的LPPQ基因序列设计引物，用Pyrobest^TM高保真DNA聚合酶从MmmSC HVRI X株中扩增出了LPPQ N末端基因序列，并进行了克隆与序列测定。核苷酸序列比较结果显示，HVRI X株的LPPQ N末端基因序列与国外发表的序列同源性为99．7％，由其推导的氨基酸序列同源性为99，1％，为脂蛋白LPPQ N末端基因体外表达奠定了基础。

Cloning and Sequence Analysis for the N-terminal Domain of the Lipoprotein LppQ of Mycoplasma mycoide Subsp.Mycoides SC

The lipoprotein LppQ gene is specific to M.mycoides subsp.mycoides SC(MmmSC) and was found in field strains isolated in Europe,Africa,as well as in vaccinal strains.The LppQ N-terminal domain possess the strong immunogenicity and induce a strong,specific,early,and persistent immune response in naturally and experimentally infected cattle.The LppQ N-terminal gene was amplified from MmmSC HVRI X strain by PCR using the primer pair designed according to the reported MmmSC LppQ sequence.It was cloned and sequenced subsequently.The result revealed that there was 99.7% identical in nucleotide sequence,99.1% identical in induced amino acid sequence,respectively.Molecular cloning of MmmSC LppQ N-terminal gene makes it possible to express the LppQ N-terminal gene in vitro.