| 快速粗提取致病疫霉菌菌丝DNA的方法 |
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| 引用本文： | 李海娟,孙银银,权军利,单卫星.快速粗提取致病疫霉菌菌丝DNA的方法[J].北方园艺,2010(17):168-170. |
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| 作者姓名： | 李海娟 孙银银 权军利 单卫星 |
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| 作者单位： | 西北农林科技大学,植物保护学院,陕西,杨凌,712100;西北农林科技大学,植物保护学院,陕西,杨凌,712100;西北农林科技大学,植物保护学院,陕西,杨凌,712100;西北农林科技大学,植物保护学院,陕西,杨凌,712100 |
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| 基金项目： | 国家公益性行业科研专项资助项目，现代农业产业技术体系资助项目 |
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| 摘 要： | 为满足规模分析病菌群体、认识病菌的群体遗传变异特征,以致病疫霉菌(Phytoph-thorainfestans)为材料,建立快速、简便的用于PCR扩增的病菌基因组DNA。分别大量和微量提取基因组DNA,比较2种方法所提取的DNA用于SSR标记的PCR扩增效果。结果表明:以微量粗提法所得的病菌基因组DNA质量足以用于PCR扩增,与大量提取所得的DNA的PCR扩增非常一致,并且粗提的DNA与大提的DNA一样都可以在-20℃条件下保存。基于PCR扩增的遗传标记广泛用于病菌群体的分析,该研究建立的基于玻璃珠振荡法提取疫霉菌菌丝基因组DNA的微量提取方法需材少、简便且快速有效,为规模分析疫霉菌群体奠定了基础。
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| 关 键 词： | 疫霉菌 基因组DNA PCR扩增 |
Rapid Coarse DNA Extraction Method for Phytophthora infestans Mycelia |
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| LI Hai-juan,SUN Yin-yin,QUAN Jun-li,SHAN Wei-xing.Rapid Coarse DNA Extraction Method for Phytophthora infestans Mycelia[J].Northern Horticulture,2010(17):168-170. |
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| Authors: | LI Hai-juan SUN Yin-yin QUAN Jun-li SHAN Wei-xing |
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| Institution: | LI Hai-juan,SUN Yin-yin,QUAN Jun-li,SHAN Wei-xing (College of Plant Protection,Northwest Agricultural and Forestry University,Yangling,Shaanxi 712100) |
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| Abstract: | To facilitate large scale analysis and understanding of genetic variation in pathogen populations using PCR-based molecular markers,aquick,simple procedure was developed for extracting genomic DNA of Phytophthorainfestans from small amount of mycelial tissues.Genomic DNA was extracted using urea-based large-scale procedure and the developed mini-scale protocol,and the quality was compared by PCR amplification of selected SSR markers.The results showed that there was no significant difference for PCR amplifi... |
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| Keywords: | Phytophthora genomic DNA PCR amplification |
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