川产白芷遗传多样性的ISSR分析

采用ISSR标记技术对主要来自川白芷道地主产区(四川遂宁)各乡镇的川白芷材料进行遗传多样性检测。从35个ISSR引物中共筛选出15个多态性明显、反应稳定的引物,在16份供试材料DNA中共扩增出99条谱带,其中多态性条带38条,占38%。材料间ISSR标记遗传相似性系数(GS)在0.821~0.994之间,各供试材料间遗传差异不大。其中,杭白芷与川白芷材料间遗传相似系数平均值大于各川白芷材料间遗传相似系数平均值。聚类分析将16份供试材料分为两大类,其中一类全部为遂宁各乡镇白芷材料;另一类包括杭白芷、四川其他地区白芷、重庆南川白芷以及2份来自遂宁涪河以南2个乡镇的白芷材料。表明聚类结果与材料的地理分布有一定关系。

Genetic Diversity of Angelicae dahuricae Accessions from Sichuan Province by Inter-simple Sequence Repeat (ISSR)

Inter-simple sequence repeat(ISSR) method was applied to detect genetic variation of Angelicae dahuricae accessions from Suining,Sichuan province.Fifteen primers were selected from 35 ISSR primers,and 99 DNA fragments were amplified from 16 samples,38 fragments among them were polymorphic(percentage of polymorphic bands was 38%).The genetic similarity among all accessions ranged from 0.821 to 0.994,which showed little genetic difference among the tested accessions.The average of genetic similarity coefficient between Angelica dahurica var.formosana and Angelica dahurica var.formosana from Sichuan province was larger than the data among Angelica dahurica var.formosana from Sichuan province.UPGMA cluster based on genetic similarity divided the accessions into two groups,one group were all from Suining,and the other were from other regions,including two samples from the south of Fu river in Suining.This showed that some relation existed between the results of UPGMA and the geographical distribution of the samples.